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Updated: Mar 29, 2026

Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts
Published on: February 20, 2012
Comparison of the Efficiency of Viral Transduction and Episomal Transfection in Human Fibroblast Reprogramming
A S Vdovin1, A Yu Lupatov1, I V Kholodenko2
1V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
Abstract:
Induced pluripotent cells were derived from adult human skin fibroblast by using two methods of reprogramming. Episomal transfection with vectors containing oriP/EBNA-1 sequence for delivery of reprogramming genes Oct4, Sox2, Klf4, L-Myc, and Lin28 proved to be more effective than viral transduction with Sendai virus-based vector: ~200 and 8 colonies per 10(5) cells were found on day 21 of culturing, respectively. Colonies of induced pluripotent cells obtained by these two methods expressed pluripotency marker Tra1-60.
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