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B lymphocyte derived hematopoietic growth factors
D H Pluznik1, M Bickel, S E Mergenhagen
1Laboratory of Immunology, National Institute of Dental Research, Bethesda, MD 20892.
Immunological Investigations
|January 1, 1989
Summary
Bacterial lipopolysaccharides (LPS) influence hematopoietic growth factor production in B cells. LPS alone induces granulocyte-macrophage colony-stimulating factor (GM-CSF) in B cell lines, suggesting post-transcriptional regulation.
Area of Science:
- Immunology
- Molecular Biology
- Hematopoiesis
Background:
- B lymphocytes are crucial immune cells involved in antibody production.
- Hematopoietic growth factors, like GM-CSF, regulate blood cell development.
- Bacterial lipopolysaccharides (LPS) are potent immune stimulators.
Purpose of the Study:
- To investigate the impact of bacterial lipopolysaccharides (LPS) on the production of hematopoietic growth factors by B lymphocytes.
- To compare GM-CSF production in primary bone marrow-derived B cells versus established B cell lines.
Main Methods:
- Utilized bone marrow-derived (BM) B cells and homogeneous B cell lines (lymphomas, hybridomas).
- Stimulated cells with LPS and/or TPA (phorbol ester) to assess GM-CSF production.
- Employed Northern analysis for mRNA levels and nuclear transcription assays for gene activity.
- Measured biological activity of GM-CSF in cell supernatants.
Main Results:
- BM B cells required both LPS and TPA for GM-CSF production.
- B cell lines produced GM-CSF upon stimulation with LPS alone.
- GM-CSF gene transcription occurred in uninduced cells, but mRNA and biological activity were only detected after LPS induction.
- Steady-state GM-CSF mRNA levels were only detectable in LPS-induced cells.
Conclusions:
- Bacterial LPS significantly influences GM-CSF production in B cells.
- B cell lines exhibit distinct responses to LPS compared to primary BM B cells.
- Regulation of GM-CSF production in response to LPS appears to occur at the post-transcriptional level.