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B lymphocyte derived hematopoietic growth factors
D H Pluznik1, M Bickel, S E Mergenhagen
1Laboratory of Immunology, National Institute of Dental Research, Bethesda, MD 20892.
The present study was undertaken to elucidate the effect of bacterial lipopolysaccharides (LPS) on B lymphocyte derived hematopoietic growth factors. Bone marrow-derived (BM) B cells and homogeneous B cell lines (lymphomas and hybridomas) were used to study the production of granulocyte-macrophage colony stimulating factor (GM-CSF). The BM B cells required two signals for GM-CSF production: LPS and a tumor promoting phorbol ester (TPA). In the B cell lines, GM-CSF production was inducible with LPS alone. Molecular analysis of the GM-CSF gene expression revealed that steady state levels (as determined by Northern analysis) of GM-CSF mRNA could be detected only in LPS-induced cells, whereas it was found by nuclear transcription analysis that the GM-CSF gene is transcribed even in uninduced cells. However, biological activity of GM-CSF was detected only in supernatants from LPS-induced cells. These data suggest a post-transcriptional regulation of this gene.
The present study was undertaken to elucidate the effect of bacterial lipopolysaccharides (LPS) on B lymphocyte derived hematopoietic growth factors. Bone marrow-derived (BM) B cells and homogeneous B cell lines (lymphomas and hybridomas) were used to study the production of granulocyte-macrophage colony stimulating factor (GM-CSF). The BM B cells required two signals for GM-CSF production: LPS and a tumor promoting phorbol ester (TPA). In the B cell lines, GM-CSF production was inducible with LPS alone. Molecular analysis of the GM-CSF gene expression revealed that steady state levels (as determined by Northern analysis) of GM-CSF mRNA could be detected only in LPS-induced cells, whereas it was found by nuclear transcription analysis that the GM-CSF gene is transcribed even in uninduced cells. However, biological activity of GM-CSF was detected only in supernatants from LPS-induced cells. These data suggest a post-transcriptional regulation of this gene.