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Quantifying Synapses: an Immunocytochemistry-based Assay to Quantify Synapse Number
Published on: November 16, 2010
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Synaptic adhesion molecule IgSF11 regulates synaptic transmission and plasticity.
Seil Jang1, Daeyoung Oh2,3, Yeunkum Lee4
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Korea.
Nature Neuroscience
|November 24, 2015
Summary
Immunoglobulin superfamily member 11 (IgSF11) is a novel synaptic protein that stabilizes AMPA glutamate receptors (AMPARs) at excitatory synapses. IgSF11 is crucial for synaptic transmission and plasticity, interacting with PSD-95 and AMPARs.
Area of Science:
- Neuroscience
- Molecular Biology
- Synaptic Plasticity
Background:
- Synaptic adhesion molecules are vital for synapse development and plasticity.
- These molecules mediate trans-synaptic adhesion and recruit synaptic proteins.
Purpose of the Study:
- To investigate the role of immunoglobulin superfamily member 11 (IgSF11) in synaptic function.
- To elucidate the interaction of IgSF11 with postsynaptic density protein 95 (PSD-95) and AMPA glutamate receptors (AMPARs).
Main Methods:
- Investigated IgSF11's binding partners and synaptic localization.
- Utilized IgSF11 knockdown and deletion in mice models.
- Employed high-throughput, single-molecule tracking to assess AMPAR surface mobility.
- Measured AMPAR-mediated synaptic transmission and long-term potentiation.
Main Results:
- IgSF11 binds to both PSD-95 and AMPARs, requiring PSD-95 for synaptic localization.
- IgSF11 stabilizes synaptic AMPARs, suppressing their surface mobility.
- IgSF11 deletion impairs synaptic transmission and hippocampal long-term potentiation.
- IgSF11 does not affect AMPAR functional characteristics like desensitization.
Conclusions:
- IgSF11 acts as a crucial regulator of excitatory synaptic transmission and plasticity.
- Its function is mediated through tripartite interactions with PSD-95 and AMPARs.
- IgSF11 represents a potential therapeutic target for neurological disorders affecting synaptic function.
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