Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hydrogen Bonds01:04

Hydrogen Bonds

16.2K
A hydrogen bond is formed when a weakly positive hydrogen atom already bonded to one electronegative atom (for example, the oxygen in the water molecule) is attracted to another electronegative atom from another polar molecule, such as water (H2O), hydrogen fluoride (HF), or ammonia (NH3). The huge electronegativity difference between the H atom (2.1) and the atom to which it is bonded (4.0 for an F atom, 3.5 for an O atom, or 3.0 for an N atom), combined with the very small size of an H atom...
16.2K
Hydrogen Bonds00:26

Hydrogen Bonds

136.3K
Hydrogen bonds are weak attractions between atoms that have formed other chemical bonds. One of these atoms is electronegative, like oxygen, and has a partial negative charge. The other is a hydrogen atom that has bonded with another electronegative atom and has a partial positive charge.
Hydrogen Bonds Control the World!
Because hydrogen has very weak electronegativity when it binds with a strongly electronegative atom, such as oxygen or nitrogen, electrons in the bond are unequally shared....
136.3K
Hess's Law03:40

Hess's Law

57.5K
There are two ways to determine the amount of heat involved in a chemical change: measure it experimentally, or calculate it from other experimentally determined enthalpy changes. Some reactions are difficult, if not impossible, to investigate and make accurate measurements for experimentally. And even when a reaction is not hard to perform or measure, it is convenient to be able to determine the heat involved in a reaction without having to perform an experiment.
57.5K
Hybridization of Atomic Orbitals II03:35

Hybridization of Atomic Orbitals II

50.3K
sp3d and sp3d 2 Hybridization
50.3K
Introduction to Chemical Bonds01:01

Introduction to Chemical Bonds

13.6K
Chemical Bonds
The electrons of the outermost energy level determine the energetic stability of the atom and its tendency to form chemical bonds with other atoms. The innermost electron shell has a maximum capacity of two electrons, but the next two electron shells can each have a maximum of eight electrons. This is known as the octet rule, which states that, with the exception of the innermost shell, atoms are most stable energetically when they have eight electrons in their valence shell, the...
13.6K
Energy to Drive Translocation01:37

Energy to Drive Translocation

3.0K
Mitochondrial protein import is powered by two distinct energy sources: ATP hydrolysis and electrochemical potential across the inner membrane. Newly synthesized precursors are bound by cytosolic chaperones of the Hsp70 family, which guide them to the import receptors on the mitochondrial surface. Utilizing the energy of ATP hydrolysis, Hsp70 chaperones transfer these precursors to the TOM receptors on the mitochondrial outer membrane.
Generally, polypeptides are unfolded by two distinct...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DDLVis: Real-time Visual Query of Spatiotemporal Data Distribution via Density Dictionary Learning.

IEEE transactions on visualization and computer graphics·2021
Same author

Silicone gel sheeting for treating hypertrophic scars.

The Cochrane database of systematic reviews·2021
Same author

DAB2IP decreases cell growth and migration and increases sensitivity to chemotherapeutic drugs in colorectal cancer.

Annals of translational medicine·2021
Same author

Parametric comparison between sparsity-based and deep learning-based image reconstruction of super-resolution fluorescence microscopy.

Biomedical optics express·2021
Same author

Programmable dual responsive system reconstructing nerve interaction with small-diameter tissue-engineered vascular grafts and inhibiting intimal hyperplasia in diabetes.

Bioactive materials·2021
Same author

ProtRe-CN: Protein Remote Homology Detection by Combining Classification Methods and Network Methods via Learning to Rank.

IEEE/ACM transactions on computational biology and bioinformatics·2021

Related Experiment Video

Updated: Mar 29, 2026

A Hydrogen-Deuterium Exchange Mass Spectrometry HDX-MS Platform for Investigating Peptide Biosynthetic Enzymes
11:32

A Hydrogen-Deuterium Exchange Mass Spectrometry HDX-MS Platform for Investigating Peptide Biosynthetic Enzymes

Published on: May 4, 2020

8.8K

H1 provides the missing link.

Michael S Y Huen1,2, Junjie Chen3

  • 1School of Biomedical Sciences, The University of Hong Kong, Hong Kong SAR.

Cell Research
|November 25, 2015
PubMed
Summary

Researchers discovered non-degradative ubiquitin marks on histone H1. These marks act as crucial signaling intermediates in the DNA damage response pathway.

More Related Videos

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1
11:27

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

Published on: September 18, 2019

10.1K
Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors
16:49

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors

Published on: July 16, 2012

12.1K

Related Experiment Videos

Last Updated: Mar 29, 2026

A Hydrogen-Deuterium Exchange Mass Spectrometry HDX-MS Platform for Investigating Peptide Biosynthetic Enzymes
11:32

A Hydrogen-Deuterium Exchange Mass Spectrometry HDX-MS Platform for Investigating Peptide Biosynthetic Enzymes

Published on: May 4, 2020

8.8K
Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1
11:27

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

Published on: September 18, 2019

10.1K
Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors
16:49

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors

Published on: July 16, 2012

12.1K

Area of Science:

  • Molecular Biology
  • Epigenetics
  • DNA Damage Response

Background:

  • The Histone Code describes epigenetic modifications regulating gene expression.
  • DNA double-strand breaks (DSBs) trigger complex cellular responses.
  • Histone modifications play a role in DNA repair signaling.

Purpose of the Study:

  • To investigate the role of linker histone H1 in the DNA damage response.
  • To identify novel signaling intermediates in the DNA damage cascade.

Main Methods:

  • Utilized proteomic analysis to identify histone modifications.
  • Employed biochemical assays to study histone H1 ubiquitination.
  • Investigated the functional consequences of H1 ubiquitination in DSB signaling.

Main Results:

  • Identified non-degradative ubiquitin marks on linker histone H1.
  • Demonstrated that these ubiquitin marks function as key signaling intermediates.
  • Showcased the involvement of H1 ubiquitination in the DNA damage signal transduction cascade.

Conclusions:

  • Non-degradative ubiquitination of histone H1 is a novel component of the Histone Code.
  • Histone H1 ubiquitination serves as a critical signaling mechanism in response to DNA double-strand breaks.