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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

101.0K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
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Genomics02:02

Genomics

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Related Experiment Video

Updated: Mar 29, 2026

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
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Advances, practice, and clinical perspectives in high-throughput sequencing.

S-J Park1, M Saito-Adachi2, Y Komiyama1

  • 1Human Genome Center, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.

Oral Diseases
|November 26, 2015
PubMed
Summary
This summary is machine-generated.

High-throughput sequencing technologies offer powerful insights into genetic and epigenetic factors influencing human health and disease. This review details platform characteristics, applications, bioinformatics tools, and clinical perspectives, particularly for oral health.

Keywords:
bioinformaticshigh-throughput sequencingoral health and diseasestranscriptome analysis

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Area of Science:

  • Genomics and Molecular Biology
  • Bioinformatics and Computational Biology

Background:

  • High-throughput sequencing technologies have revolutionized the understanding of genetic and epigenetic underpinnings of human health and disease.
  • These advancements necessitate a comprehensive evaluation of sequencing platforms, global trends, benefits, and limitations.

Purpose of the Study:

  • To review major achievements and performances of high-throughput sequencing (HTS) technologies.
  • To summarize HTS applications, including whole genome/transcriptome sequencing, genome modification identification, and protein interaction profiling.
  • To introduce applicable bioinformatics tools for big data analysis and describe a practical transcriptome analysis pipeline.

Main Methods:

  • Review of current high-throughput sequencing platforms and their global trends.
  • Summary of diverse applications in molecular biology and disease research.
  • Introduction of bioinformatics tools and a step-by-step transcriptome analysis procedure.

Main Results:

  • HTS technologies provide extensive applications for genomic and transcriptomic analysis.
  • Development of bioinformatics tools is crucial for managing and analyzing large datasets generated by HTS.
  • A practical pipeline for transcriptome analysis is presented.

Conclusions:

  • High-throughput sequencing offers significant benefits for understanding human health and disease.
  • Effective bioinformatics strategies are essential for maximizing the utility of HTS data.
  • Clinical perspectives, especially in human oral health, are highlighted.