Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.8K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

1.9K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
1.9K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

999
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
999

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dual Action Spectroscopy Exposes the Bright and Dark Excitons of Room-Temperature WSe<sub>2</sub>.

Nano letters·2025
Same author

Spatiotemporal Exciton Tracking with a SPAD Camera.

ACS photonics·2025
Same author

Ultrabroadband Optical Diffraction Tomography.

ACS photonics·2024
Same author

Photoelectrochemical Two-Dimensional Electronic Spectroscopy (PEC2DES) of Photosystem I: Charge Separation Dynamics Hidden in a Multichromophoric Landscape.

ACS applied materials & interfaces·2024
Same author

Fisher information for smart sampling in time-domain spectroscopy.

The Journal of chemical physics·2024
Same author

Structured Excitation Energy Transfer: Tracking Exciton Diffusion below Sunlight Intensity.

ACS photonics·2024
Same journal

Charge localization, rectification, and transport in electrolyte patchy nanochannels.

Faraday discussions·2026
Same journal

Ambient stability and surface adhesion of 2D polyaramid nanofilms.

Faraday discussions·2026
Same journal

Spiers Memorial Lecture: Spin-mediated promotion of magnetic metal catalysts.

Faraday discussions·2026
Same journal

Helium spin-echo as a surface-sensitive probe of vibrational energy dissipation.

Faraday discussions·2026
Same journal

Near-infrared vibrational second harmonic generation: a new nonlinear interfacial vibrational spectroscopy.

Faraday discussions·2026
Same journal

CO on a Rh/Fe<sub>3</sub>O<sub>4</sub> single-atom catalyst: high-resolution infrared spectroscopy and near-ambient-pressure scanning tunnelling microscopy.

Faraday discussions·2026
See all related articles

Related Experiment Video

Updated: Mar 29, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.4K

Single molecule microscopy and spectroscopy: concluding remarks.

Niek F van Hulst1

  • 1ICFO - Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, Barcelona and ICREA - Institució Catalana de Recerca i Estudis Avançats, Barcelona, Spain. niek.vanhulst@icfo.eu.

Faraday Discussions
|November 26, 2015
PubMed
Summary
This summary is machine-generated.

Single Molecule Detection offers a revolutionary way to observe molecules in action under ambient conditions, advancing chemistry, physics, and biology. This breakthrough technology provides unprecedented insights into molecular behavior and reactions.

More Related Videos

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
20:00

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

14.5K
High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
11:24

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

Published on: May 13, 2017

11.4K

Related Experiment Videos

Last Updated: Mar 29, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.4K
Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
20:00

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

14.5K
High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
11:24

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

Published on: May 13, 2017

11.4K

Area of Science:

  • Chemistry
  • Physics
  • Biology

Background:

  • Traditional chemistry relies on theoretical models and macroscopic observations.
  • Visualizing molecules in real-time at ambient conditions has been a long-standing scientific challenge.
  • Michael Faraday's early experiments hinted at electrical forces governing atomic interactions.

Purpose of the Study:

  • To explore the advancements and applications of Single Molecule Detection.
  • To discuss the impact of Single Molecule Microscopy and Spectroscopy in scientific research.
  • To summarize key findings and future perspectives presented at the Faraday Discussion.

Main Methods:

  • Single Molecule Detection techniques
  • Single Molecule Microscopy
  • Spectroscopy

Main Results:

  • Single Molecule Detection enables direct observation of molecules in action.
  • This technology provides a deeper understanding of molecular dynamics and reactions.
  • The Faraday Discussion highlighted significant progress in the field.

Conclusions:

  • Single Molecule Detection is a transformative technology with broad implications across scientific disciplines.
  • Continued research in this area promises further breakthroughs in understanding molecular phenomena.
  • The field is rapidly evolving, offering new avenues for scientific discovery.