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Yeast As a Chassis for Developing Functional Assays to Study Human P53
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Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

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p53 siRNA - a therapeutic tool with significant implication in the modulation of apoptosis and angiogenic pathways

Ovidiu Braicu1, Valentina Pileczki2, Cornelia Braicu2

  • 1Department of Surgery, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania ; Research Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania ; Department of Surgical Oncology, The Oncological Institute Prof. Dr. Ion Chiricuta, Cluj-Napoca, Romania.

Clujul Medical (1957)
|November 27, 2015
PubMed
Abstract

Insights

RNA interference (RNAi) therapy targeting mutated p53 demonstrated reduced cancer cell migration and invasion. This approach downregulates key genes in apoptosis and angiogenesis, offering a promising strategy for various cancers.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Therapy

Background:

  • Small interfering RNAs (siRNAs) offer novel cancer therapeutic potential by targeting oncogenes or mutated tumor suppressor genes.
  • Mutated p53 can gain oncogenic functions, making it a target for therapeutic intervention.

Purpose of the Study:

  • To investigate the role of p53 using RNA interference (RNAi) in a HeLa cell culture model.
  • To assess the impact of p53 siRNA therapy on cancer cell migration, invasion, and gene expression related to apoptosis and angiogenesis.

Main Methods:

  • Utilized an in vitro HeLa cell culture model.
  • Assessed cell migration using xCELLigence Systems.
  • Quantified gene expression of apoptosis- and angiogenesis-related genes, with protein-level validation.

Main Results:

  • p53 siRNA therapy correlated with reduced cell migration in the in vitro model.
  • Observed downregulation of p53, PTEN, TNFα, NFkB, BCL-2, ICAM-2, VEGF, and FGFb following p53 inhibition.

Conclusions:

  • RNAi technology shows potential for restoring normal cellular phenotype.
  • Targeting p53 with specific inhibitors may enhance therapeutic responses across a broad spectrum of tumoral pathologies beyond cervical cancer.
Keywords:
angiogenesisapoptosiscancerp53 siRNA

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