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Microarray Technology and Its Applications for Detecting Plasma microRNA Biomarkers in Digestive Tract Cancers

Hirotaka Konishi1, Daisuke Ichikawa2, Tomohiro Arita2

  • 1Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, Kyoto, Japan. h-koni7@koto.kpu-m.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)
|November 29, 2015
PubMed

Keywords:
Digestive tract cancerLiquid biopsyPlasma biomarkermiRNA microarray

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DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...