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Related Concept Videos

Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
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Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
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Updated: Mar 29, 2026

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
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Advances in discovering small molecules to probe protein function in a systems context.

Shelby K Doyle1, Marius S Pop1, Helen L Evans1

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High throughput screening (HTS) is now widely used in academic research for discovering chemical probes. New HTS methods are expanding beyond traditional drug discovery targets.

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Area of Science:

  • Biochemistry
  • Pharmacology
  • Chemical Biology

Background:

  • High throughput screening (HTS) was traditionally limited to the pharmaceutical industry for drug discovery.
  • Decreasing costs and rising interest in biomolecular probes have driven HTS adoption in academic settings.
  • This has spurred the development of novel HTS methodologies.

Purpose of the Study:

  • To review recent advancements in high throughput screening technologies.
  • To highlight new methods for chemical probe discovery.
  • To discuss the expansion of HTS beyond classical drug discovery.

Main Methods:

  • Review of emerging HTS technologies.
  • Analysis of novel methodologies for probe discovery.
  • Comparison with traditional HTS approaches.

Main Results:

  • HTS is now integral to academic and non-profit research institutions.
  • New HTS methods broaden target classes and capabilities.
  • Innovations facilitate the discovery of chemical probes for various biomolecules.

Conclusions:

  • HTS has democratized chemical probe discovery.
  • Advanced HTS techniques are crucial for understanding biological processes.
  • The field is rapidly evolving beyond traditional pharmaceutical applications.