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AMPK: An Energy-Sensing Pathway with Multiple Inputs and Outputs.
D Grahame Hardie1, Bethany E Schaffer2, Anne Brunet2
1Division of Cell Signalling and Immunology, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK.
Trends in Cell Biology
|December 1, 2015
Summary
AMP-activated protein kinase (AMPK) regulates cellular energy balance. This review covers AMPK activation, its role in energy homeostasis, and methods to identify its substrates and recognition motif.
Area of Science:
- Biochemistry
- Cell Biology
- Metabolic Regulation
Background:
- AMP-activated protein kinase (AMPK) is a crucial enzyme for maintaining cellular energy balance.
- It is ubiquitously expressed in eukaryotic cells and plays a vital role in energy homeostasis.
Purpose of the Study:
- To review the canonical and noncanonical mechanisms of AMPK activation.
- To discuss methods for identifying AMPK substrates and its target recognition motif.
Main Methods:
- Literature review of canonical and noncanonical AMPK activation pathways.
- Overview of hypothesis-driven and unbiased approaches for substrate identification.
- Focus on methods for determining the AMPK target recognition motif.
Main Results:
- AMPK activation restores energy homeostasis by promoting ATP-generating catabolic pathways and inhibiting ATP-consuming anabolic pathways.
- Various methods have identified numerous AMPK substrates involved in metabolic and non-metabolic processes.
- The AMPK target recognition motif can be identified and used for predicting new substrates.
Conclusions:
- Understanding AMPK activation and substrate identification is key to metabolic research.
- The identified target recognition motif aids in predicting novel AMPK substrates.
- This review provides insights into AMPK's role in cellular energy regulation.
Keywords:
AMPKallosteric activationenergy sensingkinase recognition motifkinase target identificationpharmacological activatorsMore Related Videos
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