Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

College students' life stress and internet addiction: the roles of loneliness and physical exercise.

Frontiers in psychology·2026

Same author

The influence of physical exercise on college students' prosocial behavior: the chain mediating role of social conscientiousness and general self-efficacy.

Frontiers in psychology·2026

Same author

The role of ultrasound in addressing neurodegenerative diseases: A review of mechanisms, applications, and challenges.

Neuroscience·2026

Same author

Knowledge, attitudes, and practices toward obstructive sleep apnea among high-risk populations.

BMC public health·2026

Same author

Robot steering-angle prediction lightweight network based non-local attention and lane guidance.

PloS one·2026

Same author

The effects of transcranial direct current stimulation on EEG power and complexity of ischemic stroke.

Frontiers in neuroscience·2025

Search research articles

Related Experiment Video

Updated: Mar 29, 2026

A Quantitative Glycomics and Proteomics Combined Purification Strategy

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

QUANTITY: An Isobaric Tag for Quantitative Glycomics.

Shuang Yang¹, Meiyao Wang^2,3, Lijun Chen¹

¹Department of Pathology, Johns Hopkins University, Baltimore, MD, USA.

Scientific Reports

|December 1, 2015

Summary

This summary is machine-generated.

We developed QUANTITY (Quaternary Amine Containing Isobaric Tag for Glycan), a new method for high-throughput glycomic analysis. This technique enhances glycan detection by mass spectrometry, aiding glycan analysis and bioengineering applications.

More Related Videos

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis QDB

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis QDB

Published on: August 21, 2018

Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System

Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System

Published on: June 28, 2024

Related Experiment Videos

Last Updated: Mar 29, 2026

A Quantitative Glycomics and Proteomics Combined Purification Strategy

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis QDB

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis QDB

Published on: August 21, 2018

Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System

Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System

Published on: June 28, 2024

Area of Science:

Biochemistry
Analytical Chemistry
Biotechnology

Background:

Glycans are crucial macromolecules with diverse biological roles.
Quantitative glycomics lags behind genomics and proteomics due to technical challenges.
Advanced technologies for global glycan analysis are essential.

Purpose of the Study:

To introduce QUANTITY (Quaternary Amine Containing Isobaric Tag for Glycan), a novel quantitative approach for glycomic analysis.
To enhance glycan detection and enable high-throughput analysis using mass spectrometry.
To facilitate glycomic surveys of complex biological samples, including engineered cells.

Main Methods:

Development of QUANTITY, a quaternary amine-containing isobaric tag.
Application of mass spectrometry for enhanced glycan detection.
High-throughput glycomic analysis of multiple biological samples.

Main Results:

QUANTITY significantly enhances glycan detection sensitivity.
The method enables high-throughput glycomic analysis from multiple samples.
Successful glycomic survey of bioengineered Chinese Hamster Ovary (CHO) cells was achieved.

Conclusions:

QUANTITY is a robust and invaluable tool for advanced glycan analysis.
This technique supports glycomic surveys in bioengineering contexts.
The approach aids in understanding protein glycosylation in engineered systems.