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Measurement of proteins with the Behring Nephelometer. A multicentre evaluation
1Institut für LaboratoriumsmedizinZentrallabor, Zentralkrankenhaus St.-Jürgen-Strasse, Bremen, Federal Republic of Germany.
Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
|April 1, 1989
Summary
The Behring Nephelometer demonstrates high precision and reliability for clinical laboratory analysis, showing excellent performance in multi-protein measurements across various sample types. This automated analyzer is suitable for routine and emergency diagnostics.
Area of Science:
- Clinical Chemistry
- Immunology
- Laboratory Medicine
Background:
- Accurate quantification of serum and cerebrospinal fluid proteins is crucial for diagnosing various medical conditions.
- The Behring Nephelometer is a selective multi-protein analyzer designed for high-throughput laboratory diagnostics.
Purpose of the Study:
- To evaluate the performance of the Behring Nephelometer according to ECCLS guidelines.
- To assess the analyzer's precision, accuracy, and reliability in a multicenter setting.
Main Methods:
- A multicenter evaluation involving five laboratories and over 20,000 analyses.
- Measurement of immunoglobulins (IgG, IgA, IgM), C-reactive protein, complement factors (C3c, C4), apolipoproteins (A-I, B), and albumin.
- Comparison with established analytical methods and assessment of interferences and carry-over effects.
Main Results:
- High precision with between-day coefficients of variation generally below 4.5%.
- Good agreement with comparative methods and acceptable control material values (0.95-1.08).
- No carry-over or significant interferences observed, except for lipaemic samples and some monoclonal IgM.
Conclusions:
- The Behring Nephelometer exhibits excellent reliability and precision for routine and emergency analyses.
- Its wide measuring range minimizes the need for repeat analyses.
- The analyzer is well-suited for use in medium to large clinical laboratories.