Somatic Embryogenesis Induction and Plant Regeneration in Strawberry Tree (Arbutus unedo L.)
João F Martins1, Sandra I Correia1, Jorge M Canhoto2
1Center for Functional Ecology, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, Apartado 3046, 3001-401, Coimbra, Portugal.
Abstract:
Somatic embryogenesis is a powerful tool both for cloning and studies of genetic transformation and embryo development. Most protocols for somatic embryogenesis induction start from zygotic embryos or embryonic-derived tissues which do not allow the propagation of elite trees. In the present study, a reliable protocol for somatic embryogenesis induction from adult trees of strawberry tree is described. Leaves from in vitro proliferating shoots were used to induce somatic embryo formation on a medium containing an auxin and a cytokinin. Somatic embryos germinated in a plant growth regulator-free medium.
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