Advenella alkanexedens sp. nov., an alkane-degrading bacterium isolated from biogas slurry samples

A novel aerobic bacterium, designated strain LAM0050^T, was isolated from a biogas slurry sample, which had been enriched with diesel oil for 30 days. Cells of strain LAM0050^T were gram-stain-negative, non-motile, non-spore-forming and coccoid-shaped. The optimal temperature and pH for growth were 30-35 °C and 8.5, respectively. The strain did not require NaCl for growth, but tolerated up to 5.3 % (w/v) NaCl. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain LAM0050^T was a member of the genus Advenella, and was most closely related to Advenella faeciporci KCTC 23732^T, Advenella incenata CCUG 45225^T, Advenella kashmirensis DSM 17095^T and Advenella mimigardefordensis DSM 17166^T, with 98.1, 96.6, 96.6 and 96.3 % sequence similarity, respectively. The DNA-DNA hybridization relatedness between strain LAM0050^T and A. faeciporci KCTC 23732^T was 41.7 ± 2.4 %. The genomic DNA G+C content was 51.2 mol%, as determined by the T_m method. The major fatty acids of strain LAM0050^T were C_16 : 0, C_17 : 0 cyclo, summed feature 3 (C_16 : 1ω7c and/or C_16 : 1ω6c) and summed feature 8 (C_18 : 1ω7c and/or C_18 : 1ω6c). The predominant ubiquinone was Q-8. The main polar lipids were diphosphatidyglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine and four unidentified phospholipids. Based on the phenotypic and genotypic properties, strain LAM0050^T is suggested to represent a novel species of the genus Advenella, for which the name Advenella alkanexedens sp. nov., is proposed, the type strain is LAM0050^T ( = ACCC 06485^T = JCM 30465^T).