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MicroRNA-18a regulates invasive meningiomas via hypoxia-inducible factor-1α

Puxian Li1, Yong Gao1, Fengjia Li1

  • 1Department of Neurosurgery, Laiwu City People's Hospital, Laiwu, Shandong 271100, P.R. China.

Experimental and Therapeutic Medicine
|December 2, 2015
PubMed

Insights

MicroRNA-18a (miR-18a) is downregulated in invasive meningiomas, leading to increased hypoxia-inducible factor-1α (HIF-1α) expression. This suggests miR-18a may inhibit meningioma invasion and metastasis by regulating HIF-1α.

Area of Science:

  • Neuro-oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Meningiomas are primary tumors of the central nervous system, with invasive subtypes posing significant clinical challenges.
  • Understanding the molecular mechanisms driving meningioma invasiveness and metastasis is crucial for developing targeted therapies.

Purpose of the Study:

  • To investigate the role of microRNA-18a (miR-18a) in the invasiveness and metastasis of invasive meningiomas.
  • To elucidate the underlying molecular mechanism involving hypoxia-inducible factor-1α (HIF-1α).

Main Methods:

  • Quantitative analysis of miR-18a and HIF-1α mRNA expression using reverse transcription-quantitative polymerase chain reaction (RT-qPCR).
  • Protein expression levels of HIF-1α were determined by Western blot analysis.
  • Samples included meningioma tissues, serum, and cerebrospinal fluid from patients and controls.

Main Results:

  • Hypoxia-inducible factor-1α (HIF-1α) mRNA and protein levels were significantly elevated in invasive meningioma tissues, serum, and cerebrospinal fluid compared to controls.
  • MicroRNA-18a (miR-18a) expression was significantly reduced in invasive meningiomas compared to non-invasive and control groups.
  • A negative correlation was observed between miR-18a expression and HIF-1α levels, suggesting miR-18a downregulates HIF-1α.

Conclusions:

  • Hypoxia-inducible factor-1α (HIF-1α) is upregulated in invasive meningiomas, potentially driven by the downregulation of microRNA-18a (miR-18a).
  • MicroRNA-18a (miR-18a) may function as a tumor suppressor by inhibiting meningioma invasiveness and metastasis through the regulation of HIF-1α.
Keywords:
hypoxia-inducible factor-1αinvasivenessmeningiomamicroRNA-18a

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