Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.8K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.8K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

2.3K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.3K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

2.3K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
2.3K
Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

2.4K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
2.4K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

28.6K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.6K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

4.1K
4.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Feeder-free yet still naïve: improved method for capturing human pluripotent stem cells.

The EMBO journal·2026
Same author

Post-replicative chromatin accessibility predicts cell fate change.

Stem cell reports·2026
Same author

Morphomechanic tuning of ERK by actin-TFII-IΔ regulates cell identity.

bioRxiv : the preprint server for biology·2026
Same author

Modulation of Nudt21 levels reveals dose-dependent roles of alternative polyadenylation in tissue regeneration.

Nature communications·2026
Same author

Metformin inhibits nuclear egress of chromatin fragments in senescence and aging.

Nature aging·2026
Same author

Systematic characterization of existing and novel inducible transgenic systems in human pluripotent stem cells after prolonged differentiation.

bioRxiv : the preprint server for biology·2025
Same journal

Evolutionary and Biochemical Perspectives on the Incorporation and Utilization of Selenocysteine.

Cold Spring Harbor perspectives in biology·2026
Same journal

The Mitochondrial Calcium Uniporter: From Parts to Signaling Networks.

Cold Spring Harbor perspectives in biology·2026
Same journal

Growth Control and Beyond: Functional Diversity and Regulation of the Hippo Pathway in the Nervous System.

Cold Spring Harbor perspectives in biology·2026
Same journal

Structural Studies of Core Hippo Pathway Components.

Cold Spring Harbor perspectives in biology·2026
Same journal

The Hippo Pathway in Intestinal Regeneration, Fetal Reprogramming, and Tumorigenesis.

Cold Spring Harbor perspectives in biology·2026
Same journal

A Synergy between Genetics and Biochemistry Unravels the Molecular Architecture of the Hippo Signaling Pathway.

Cold Spring Harbor perspectives in biology·2026
See all related articles

Related Experiment Video

Updated: Mar 29, 2026

Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets
07:08

Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets

Published on: February 2, 2024

1.6K

Induced Pluripotency and Epigenetic Reprogramming.

Konrad Hochedlinger1, Rudolf Jaenisch2

  • 1Howard Hughes Medical Institute at Massachusetts General Hospital, Department of Stem Cell and Regenerative Biology, Harvard University and Harvard Medical School, Boston, Massachusetts 02114.

Cold Spring Harbor Perspectives in Biology
|December 3, 2015
PubMed
Summary
This summary is machine-generated.

Induced pluripotency converts somatic cells into induced pluripotent stem cells (iPSCs) using transcription factors. This review covers iPSC generation, reprogramming mechanisms, and therapeutic applications, including genome engineering.

More Related Videos

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
11:38

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

Published on: November 26, 2018

11.2K
Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
10:32

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

Published on: September 6, 2014

12.8K

Related Experiment Videos

Last Updated: Mar 29, 2026

Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets
07:08

Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets

Published on: February 2, 2024

1.6K
RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
11:38

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

Published on: November 26, 2018

11.2K
Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
10:32

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

Published on: September 6, 2014

12.8K

Area of Science:

  • Stem cell biology
  • Epigenetics
  • Molecular biology

Background:

  • Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs).
  • This process, termed induced pluripotency, is driven by specific transcription factors.
  • Understanding iPSCs is crucial for regenerative medicine and disease modeling.

Purpose of the Study:

  • To provide a historical context for transcription factor-induced pluripotency.
  • To review current methodologies for generating iPSCs.
  • To discuss mechanistic insights and therapeutic applications of iPSCs, including genome engineering.

Main Methods:

  • Literature review of induced pluripotency research.
  • Analysis of transcription factor-mediated reprogramming.
  • Examination of genome engineering techniques in pluripotent cells.

Main Results:

  • Transcription factor-induced pluripotency has a developing historical context.
  • Various methods exist for iPSC generation, each with specific efficiencies and applications.
  • Mechanistic insights into reprogramming are advancing.
  • Genome engineering technologies offer precise modification of human pluripotent cells.

Conclusions:

  • Induced pluripotency is a transformative technology with significant potential in regenerative medicine.
  • Further research into reprogramming mechanisms and therapeutic applications is warranted.
  • Efficient genome engineering of human pluripotent cells will accelerate scientific discovery and therapeutic development.