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MicroRNA-365 in macrophages regulates Mycobacterium tuberculosis-induced active pulmonary tuberculosis via
Qingzhang Song1, Hui Li2, Hua Shao1
1Department of Infectious Diseases, Laiwu City People's Hospital Laiwu, P. R. China.
Abstract:
The present study is to investigate the relationship between microRNA (miR)-365 expression and the levels of interleukin (IL)-6 mRNA and protein in patients with active tuberculosis. From June 2011 to June 2014, 48 patients with active pulmonary tuberculosis induced by Mycobacterium tuberculosis were included in the study. In addition, 23 healthy subjects were enrolled as control. Macrophages were collected by pulmonary alveolus lavage. In addition, serum and mononuclear cells were isolated from peripheral blood. The levels of miR-365 and IL-6 in macrophages, mononuclear cells and serum were determined using quantitative real-time polymerase chain reaction. The protein expression of IL-6 in macrophages and mononuclear cells was measured using Western blotting, while that in serum was detected by enzyme-linked immunoabsorbent assay. Expression of IL-6 mRNA and protein was significantly enhanced in patients with active pulmonary tuberculosis. Increase of IL-6 protein concentration in serum was probably due to the release of IL-6 protein from mononuclear cells in the blood. In addition, miR-365 levels were significantly lowered in patients with active pulmonary tuberculosis. Up-regulated IL-6 expression in macrophages, mononuclear cells and serum in patients with active pulmonary tuberculosis is related to the down-regulation of miR-365, suggesting that miR-365 may regulate the occurrence and immune responses of active pulmonary tuberculosis via IL-6.
Insights
This study found lower microRNA (miR)-365 levels in active tuberculosis patients, correlating with increased interleukin (IL)-6. This suggests miR-365 may influence tuberculosis immune responses by regulating IL-6.
Area of Science:
- Immunology
- Molecular Biology
- Biochemistry
Background:
- Tuberculosis (TB) is a significant global health challenge.
- Interleukin-6 (IL-6) is a key inflammatory cytokine implicated in TB pathogenesis.
- MicroRNAs (miRNAs) are emerging as critical regulators of immune responses.
Purpose of the Study:
- To investigate the relationship between microRNA (miR)-365 expression and interleukin (IL)-6 levels in active pulmonary tuberculosis patients.
- To explore the potential role of miR-365 in regulating IL-6 in the context of Mycobacterium tuberculosis infection.
Main Methods:
- Quantitative real-time polymerase chain reaction (qRT-PCR) to measure miR-365 and IL-6 mRNA levels.
- Western blotting and enzyme-linked immunoabsorbent assay (ELISA) to determine IL-6 protein expression.
- Analysis of samples from 48 active pulmonary tuberculosis patients and 23 healthy controls.
Main Results:
- Significantly elevated IL-6 mRNA and protein expression were observed in patients with active pulmonary tuberculosis compared to controls.
- Down-regulation of miR-365 was detected in patients with active pulmonary tuberculosis.
- Increased IL-6 protein in serum was associated with its release from mononuclear cells.
Conclusions:
- Down-regulation of miR-365 is associated with up-regulated IL-6 expression in active pulmonary tuberculosis.
- miR-365 may play a regulatory role in the occurrence and immune responses of active pulmonary tuberculosis through modulation of IL-6.
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