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19F Oximetry with semifluorinated alkanes
Stefan Kegel1, Jorge Chacon-Caldera2, Charalambos Tsagogiorgas3
1a Medical Radiation Physics/Radiation Protection, Universitätsmedizin Mannheim, Medical Faculty Mannheim, Heidelberg University , Mannheim , Germany.
Artificial Cells, Nanomedicine, and Biotechnology
|December 4, 2015
Summary
This study shows semifluorinated alkanes (SFAs) can measure oxygen tension (pO2) using 19F-MRI. SFAs offer a less oxygen-sensitive alternative to perfluorocarbons, with temperature influencing measurements more significantly.
Area of Science:
- Biomedical Engineering
- Magnetic Resonance Imaging
- Fluorine Chemistry
Background:
- Semifluorinated alkanes (SFAs) are amphiphilic, forming stable emulsions for potential clinical applications.
- Perfluorocarbons (PFCs) are commonly used but have limitations.
- 19F-MRI offers a unique window for sensing applications.
Purpose of the Study:
- To investigate the longitudinal relaxation rate (R1) of 19F-CF3 in SFAs.
- To determine the influence of oxygen tension (pO2), temperature (T), and pH on R1.
- To establish SFAs as potential contrast agents for pO2 sensing via MRI.
Main Methods:
- Measurements of R1 (1/T1) using 19F-MRI at 9.4 Tesla.
- Utilized a self-constructed birdcage resonator for fluorine-19 measurements.
- Employed standard saturation recovery sequences for T1 determination.
Main Results:
- A linear relationship between R1 and pO2 was confirmed for SFAs across various temperatures.
- SFAs demonstrated lower sensitivity to pO2 variations compared to PFCs.
- Temperature exhibited a greater influence on R1 than pO2 for SFAs.
- The effect of pH on R1 was found to be negligible.
Conclusions:
- SFAs can be calibrated to measure pO2 in vitro using 19F-MRI.
- SFAs present a viable, less oxygen-sensitive alternative to PFCs for MRI-based sensing.
- Temperature calibration is crucial for accurate pO2 measurements with SFAs.
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