Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

3.0K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
3.0K
X-ray Crystallography02:18

X-ray Crystallography

26.9K
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
26.9K
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

5.2K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
5.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structure Determination of β-Nb<sub>2</sub>N Phase in Thin Film Form by 3D Electron Diffraction.

Inorganic chemistry·2026
Same author

Nanometric mineral inclusions from a fluid-rich diamond: identification, structure, and implications for deep Earth.

Nature communications·2026
Same author

Automated serial electron diffraction: implementation in <i>LibraEDT</i> and its applications.

Journal of applied crystallography·2026
Same author

Evidence for an Electronically Driven Charge Density Wave in a 1D Metallic MOF.

ACS central science·2026
Same author

Mechanochemically assembled organometallic complexes: a mechanistic study.

Chemical science·2026
Same author

3D atomic structure determination with ultrashort-pulse MeV electron diffraction.

IUCrJ·2026

Related Experiment Video

Updated: Mar 29, 2026

Characterization of Ultra-fine Grained and Nanocrystalline Materials Using Transmission Kikuchi Diffraction
09:13

Characterization of Ultra-fine Grained and Nanocrystalline Materials Using Transmission Kikuchi Diffraction

Published on: April 1, 2017

14.3K

Structure refinement using precession electron diffraction tomography and dynamical diffraction: tests on

Lukáš Palatinus1, Cinthia Antunes Corrêa1, Gwladys Steciuk2

  • 1Institute of Physics of the AS CR, Na Slovance 2, Prague, Czech Republic.

Acta Crystallographica Section B, Structural Science, Crystal Engineering and Materials
|December 5, 2015
PubMed
Summary
This summary is machine-generated.

A new structure refinement method using dynamical diffraction theory significantly improves accuracy for electron diffraction data. This advanced technique yields more precise atomic positions and reliable site occupancies compared to older methods.

Keywords:
dynamical diffractionelectron crystallographyelectron diffraction tomographykaolinitemayeniteorthopyroxeneprecession

More Related Videos

Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments
08:31

Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments

Published on: June 27, 2022

2.4K
Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

4.2K

Related Experiment Videos

Last Updated: Mar 29, 2026

Characterization of Ultra-fine Grained and Nanocrystalline Materials Using Transmission Kikuchi Diffraction
09:13

Characterization of Ultra-fine Grained and Nanocrystalline Materials Using Transmission Kikuchi Diffraction

Published on: April 1, 2017

14.3K
Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments
08:31

Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments

Published on: June 27, 2022

2.4K
Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

4.2K

Area of Science:

  • Crystallography
  • Materials Science
  • Electron Microscopy

Background:

  • Accurate crystal structure determination is crucial for understanding material properties.
  • Traditional structure refinement methods using kinematical approximation have limitations in precision.
  • Dynamical diffraction theory offers a more rigorous approach to modeling electron diffraction intensities.

Purpose of the Study:

  • To evaluate the performance of a recently developed structure refinement method based on dynamical diffraction theory.
  • To assess the accuracy of atomic positions, site occupancies, and absolute structure determination.
  • To propose an optimized set of parameters for the method based on extensive testing.

Main Methods:

  • Application of the dynamical diffraction-based structure refinement method to experimental three-dimensional precession electron diffraction data.
  • Testing the method on five diverse crystalline samples (Ni2Si, PrVO3, kaolinite, orthopyroxene, mayenite).
  • Comparison of results with reference structures and models obtained via kinematical approximation.

Main Results:

  • The dynamical diffraction method consistently produced structure models with average errors in atomic positions between 0.01 and 0.02 Å.
  • Structure models derived from this method were demonstrably more accurate than those from kinematical refinement.
  • Reliable determination of site occupancies and absolute structure was achieved.

Conclusions:

  • The dynamical diffraction-based structure refinement method is highly effective for analyzing electron diffraction data.
  • This method offers superior accuracy and reliability for determining crystal structures, including site occupancies and absolute configurations.
  • An optimized parameter set is recommended for practical application of the method.