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Related Concept Videos

Chromosome Replication02:31

Chromosome Replication

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Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
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Replication in Eukaryotes01:29

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In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
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Overview
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Replication in Eukaryotes

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Replication in Eukaryotes01:29

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Chromosome Structure02:40

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A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
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Related Experiment Video

Updated: Mar 29, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
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Replication Origin Specification Gets a Push.

Brian S Plosky1

  • 1Molecular Cell, Cell Press, 50 Hampshire Street, 5(th) Floor, Cambridge, MA 02139, USA.

Molecular Cell
|December 7, 2015
PubMed
Summary

DNA translocases can push pre-replicative complexes (pre-RCs) along DNA without disrupting them. These pre-RCs maintain their ability to support DNA replication, ensuring cell cycle progression.

Area of Science:

  • Cellular biology
  • Molecular biology
  • Genetics

Background:

  • DNA replication is a fundamental process for cell division.
  • Pre-replicative complexes (pre-RCs) are essential for licensing DNA replication origins.
  • The G1/S phase transition involves the assembly and firing of pre-RCs.

Purpose of the Study:

  • To investigate the potential disruption of pre-RCs by DNA translocases during origin firing.
  • To determine if pre-RCs can be displaced and still support DNA replication.

Main Methods:

  • Utilizing molecular biology techniques to study protein-DNA interactions.
  • Observing the behavior of pre-RCs in the presence of DNA translocases in vitro.
  • Assessing the replication competency of displaced pre-RCs.

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Main Results:

  • DNA translocases can displace pre-RCs along the DNA molecule.
  • Displaced pre-RCs retain their structural integrity and functionality.
  • The ability of pre-RCs to support DNA replication is not compromised by translocation.

Conclusions:

  • Pre-RCs are surprisingly robust and can withstand mechanical forces from DNA translocases.
  • This finding provides new insights into the regulation of DNA replication origin firing.
  • The cell possesses mechanisms to ensure replication fidelity even when components are displaced.