Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

2.8K
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
2.8K
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

2.8K
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
2.8K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

1.8K
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
1.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lipidomic profile of meningiomas harboring different NF2 mutation status.

Metabolomics : Official journal of the Metabolomic Society·2026
Same author

Culture Dimensionality Modulates Gallium Maltolate Response in Glioblastoma: Comparative Analyses in 2D and 3D Models.

Molecular pharmaceutics·2026
Same author

Assessment of thermal and solvent stable SPME fibers for volatilome investigation through in vitro experiments with cancer cell lines.

Analytica chimica acta·2025
Same author

Profiling of Volatile Metabolites of <i>Escherichia coli</i> Using Gas Chromatography-Mass Spectrometry.

International journal of molecular sciences·2025
Same author

Minimally Invasive Chemical Biopsy Needle with Self-Wettable Extraction Phase For In Vivo Tissue Sampling During Medical Procedures.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Metabolomic Insight into Donation After Circulatory-Death Kidney Grafts in Porcine Autotransplant Model: Normothermic Ex Vivo Kidney Perfusion Compared with Hypothermic Machine Perfusion and Static Cold Storage.

International journal of molecular sciences·2025

Related Experiment Video

Updated: Mar 29, 2026

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
07:55

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

Published on: June 21, 2019

6.3K

Biocompatible Solid-Phase Microextraction Nanoelectrospray Ionization: An Unexploited Tool in Bioanalysis.

Germán Augusto Gómez-Ríos1, Nathaly Reyes-Garcés1, Barbara Bojko1

  • 1Department of Chemistry, University of Waterloo , Waterloo, Ontario N2L 3G1, Canada.

Analytical Chemistry
|December 10, 2015
PubMed
Summary
This summary is machine-generated.

Directly coupling biocompatible solid-phase microextraction (Bio-SPME) fibers to mass spectrometry offers rapid, quantitative analysis of biofluids. This method achieves low detection limits and high accuracy, integrating sample prep, cleanup, and ionization for efficient bioanalysis.

More Related Videos

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
07:54

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

Published on: October 27, 2020

5.0K
Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry
08:23

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

Published on: March 9, 2018

9.4K

Related Experiment Videos

Last Updated: Mar 29, 2026

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
07:55

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

Published on: June 21, 2019

6.3K
Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
07:54

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

Published on: October 27, 2020

5.0K
Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry
08:23

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

Published on: March 9, 2018

9.4K

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Biotechnology

Background:

  • Solid-phase microextraction (SPME) coupled with mass spectrometry (MS) offers analytical advantages.
  • Direct coupling of SPME to MS has not been fully optimized for quantitative analysis.
  • Biocompatible SPME (Bio-SPME) fibers present a novel material for biofluid analysis.

Purpose of the Study:

  • To present the direct coupling of Bio-SPME fibers to nanoelectrospray ionization (nano-ESI) mass spectrometry.
  • To establish Bio-SPME-nano-ESI as a tool for fast quantitative analysis of target analytes in biofluids.
  • To evaluate the performance of this integrated system for bioanalytical applications.

Main Methods:

  • Direct coupling of biocompatible SPME fibers to nano-ESI emitters.
  • Utilizing optimized fiber lengths and sample vessels for sample volumes from 10 to 1500 μL.
  • Performing quantitative analysis in phosphate-buffered saline (PBS), urine, and whole blood.

Main Results:

  • Achieved sub-nanogram per milliliter limits of detection with accuracy ≥90% and linearity R(2) > 0.999.
  • Demonstrated total sample preparation time not exceeding 2 minutes.
  • Successfully integrated sampling, extraction, enrichment, cleanup, and ionization in a single system.

Conclusions:

  • Bio-SPME-nano-ESI is a powerful and advantageous configuration for fast quantitative bioanalysis.
  • The technique effectively minimizes matrix effects and enhances analysis throughput.
  • Applications include therapeutic drug monitoring, doping control, and pharmacokinetic studies.