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[Klebocins K6 and K150: production and activity in various experimental conditions]
1Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Universidad Nacional de Río Cuarto, Argentina.
Abstract:
Klebsiella is a common agent in hospital-acquired infections and its importance in disease transmission was evident in the isolation obtained in a health center in the city of Rio Cuarto. Bacteriocinogenic strains by the cross-streak method in tryptic-soy agar were investigated. Two Klebsiella produced bacteriocins with broad patterns of sensitivity among the tested strains. The K150 klebocin was more active than the K6 klebocin, but this one was more heat-stable than K150. The klebocins were not detected in the synthetic media employed and they caused inhibitory areas in complex media, except in triptose-beef extract. K150 was not active in eosine-methylene blue and nutrient agar. The bacteriocins were associated to lipase activity and hemolytic effect on chicken erythrocytes. The strains 6 and 150 were multiresistant to antimicrobial agents, with a pattern of sensitivity different from that of other multiresistant strains.
Insights
Two Klebsiella strains produced bacteriocins, potent antimicrobial substances, against other hospital-acquired infection agents. These bacteriocins, K150 and K6, showed varying activity and stability, offering potential for infection control strategies.
Area of Science:
- Microbiology
- Infectious Diseases
- Bacteriology
Background:
- Klebsiella species are significant causes of hospital-acquired infections.
- Understanding Klebsiella transmission is crucial for infection control.
- Antimicrobial resistance in Klebsiella complicates treatment options.
Purpose of the Study:
- To investigate bacteriocinogenic Klebsiella strains isolated from a healthcare setting.
- To characterize the activity and properties of produced bacteriocins.
- To assess the antimicrobial resistance patterns of these strains.
Main Methods:
- Isolation of Klebsiella strains from a healthcare facility.
- Bacteriocin production and activity testing using the cross-streak method on tryptic-soy agar.
- Evaluation of bacteriocin stability under different conditions (heat, media composition).
- Determination of antimicrobial susceptibility profiles.
Main Results:
- Two Klebsiella strains (K150 and K6) produced bacteriocins with broad inhibitory activity against other tested strains.
- K150 klebocin exhibited higher activity, while K6 klebocin demonstrated greater heat stability.
- Bacteriocin activity was dependent on media composition, being detected in complex but not synthetic media.
- Associated lipase activity and hemolytic effect were observed.
- Strains 6 and 150 displayed multidrug resistance with distinct resistance patterns.
Conclusions:
- Bacteriocin-producing Klebsiella strains represent a potential avenue for combating hospital-acquired infections.
- The characterized bacteriocins (K150 and K6) possess distinct properties that warrant further investigation for therapeutic applications.
- The multidrug resistance profile of these strains highlights the ongoing challenge of antimicrobial resistance in clinical settings.