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Related Concept Videos

RNA Interference01:23

RNA Interference

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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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Types of RNA01:23

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Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
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Related Experiment Video

Updated: Mar 29, 2026

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
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Short-interference RNAs: becoming medicines.

Tamara Martínez1, Ana Isabel Jiménez1, Covadonga Pañeda1

  • 1Sylentis, R&D department c/Santiago Grisolía, Tres Cantos, Madrid, Spain.

EXCLI Journal
|December 10, 2015
PubMed
Summary

RNA interference (RNAi) is a gene-silencing mechanism. This review explores RNAi therapeutics, examining their clinical progress and potential to become mainstream medicines.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • RNA interference (RNAi) is a natural cellular process for regulating gene expression.
  • The discovery of RNAi by Fire and Mello revolutionized molecular biology.
  • RNAi technology has advanced significantly over the past decade.

Purpose of the Study:

  • To provide a comprehensive overview of the current status of RNAi technology.
  • To focus on the clinical development and therapeutic potential of RNAi-based compounds.
  • To assess the likelihood of RNAi therapeutics becoming mainstream medicines.

Main Methods:

  • Review of existing scientific literature and clinical trial data.
  • Analysis of the technological advancements in RNAi delivery and stability.
Keywords:
RNAioligonucleotidessiRNAtherapeutics

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  • Evaluation of the therapeutic applications and challenges of RNAi.

    • Main Results:

    • RNA interference is a powerful tool for modulating gene expression.
    • Numerous RNAi-based therapeutics are progressing through clinical trials.
    • Significant progress has been made in overcoming delivery and stability challenges.

    Conclusions:

    • RNAi therapeutics are on the cusp of clinical approval.
    • The coming years are critical for determining the success of RNAi-based medicines.
    • RNAi holds immense promise for treating a wide range of diseases.