A Methodological Discussion of Caffeine Research and Animal Avoidance Behavior

We present a review of the methodological aspects of caffeine research within animal tests of escape and avoidance behavior in the presence of aversive stimuli. We highlight species, methods of caffeine administration, dosage, dependent measures, and research designs commonly used in this research. Typical subjects were rodents and zebrafish, with species-specific vehicles of caffeine administration and dependent measures. Behavioral tests for escape and avoidance as a function of caffeine consumption were conceptually similar across species, although the arrangement of measures was necessarily adapted to the physiological contingencies of the different species. Caffeine administration preceding the presentation of aversive stimuli generally, but not exclusively, enhanced the effect of escape and avoidance of aversive stimuli. The many commonalities in methods and results across species suggest similar methods may be relevant to human subjects as well.