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Related Experiment Video

Updated: Mar 29, 2026

Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils
08:09

Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils

Published on: November 16, 2015

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Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils.

Farzaneh Assadian1, Karl Sandström2, Göran Laurell2

  • 1Department of Medical Biochemistry and Microbiology, Uppsala Biomedical Center, Uppsala University.

Journal of Visualized Experiments : Jove
|December 10, 2015
PubMed
Summary

This study details a method for isolating B and T lymphocytes from human palatine tonsils. The protocol efficiently separates these immune cells and demonstrates adenovirus targets T cells within tonsillar tissue.

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Area of Science:

  • Immunology
  • Virology
  • Cell Biology

Background:

  • Tonsils, particularly palatine tonsils, are crucial components of the immune system's initial defense against inhaled pathogens.
  • Surgically removed palatine tonsils offer a readily accessible source for studying host-pathogen interactions involving B and T lymphocytes.

Purpose of the Study:

  • To describe a video protocol for dissecting and processing human palatine tonsils.
  • To detail a method for isolating distinct B and T lymphocyte populations from the same tonsillar tissue sample.
  • To demonstrate the utility of the isolated cells in studying pathogen interactions, specifically human adenovirus infection of T cells.

Main Methods:

  • Dissection and processing of surgically removed human palatine tonsils.
  • Antibody-dependent affinity-based protocol for efficient separation of tonsillar B and T lymphocytes.
  • Characterization of isolated lymphocyte populations and pathogen infection studies.

Main Results:

  • An efficient method was established for separating B and T lymphocytes from human palatine tonsils.
  • The protocol successfully isolated both B and T cell populations from the same tissue sample.
  • Human adenovirus was shown to specifically infect the T cell fraction of tonsillar lymphocytes.

Conclusions:

  • The developed protocol provides an efficient and rapid means to isolate tonsillar B and T cell populations.
  • This method facilitates the study of various pathogens' roles in tonsillar immune responses.
  • The findings highlight the specific susceptibility of tonsillar T cells to human adenovirus infection.