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A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological

Erika J Fong1, Chao Huang2, Julie Hamilton2

  • 1Materials Engineering Division, Lawrence Livermore National Laboratory; Department of Biomedical Engineering, Boston University.

Journal of Visualized Experiments : Jove
|December 10, 2015
PubMed
Summary

This study introduces an automated microfluidic platform for precise particle separation from larger samples. The system ensures repeatable, sensitive results with flexible device integration and automated fluid handling for biological sample processing.

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Area of Science:

  • Biotechnology
  • Microfluidics
  • Particle Separation

Background:

  • Microfluidic devices offer advantages in sample manipulation but require robust integration with macroscale systems.
  • Achieving repeatable and sensitive particle separation necessitates addressing challenges in fluid handling and device connectivity.

Purpose of the Study:

  • To present a fully automated and integrated microfluidic platform for processing larger sample volumes (0.15-1.5 ml).
  • To enable precise and repeatable particle separation using interchangeable microfluidic devices, including acoustofluidics.
  • To optimize sample collection through real-time feedback for conservation and concentration.

Main Methods:

  • Development of a modular microfluidic platform with robust world-to-chip connections.
  • Implementation of fully-automated fluid handling for closed-loop sample collection, system priming, and cleaning.
  • Integration and characterization of an acoustofluidic device for size-based separation of biological samples.

Main Results:

  • Demonstrated precise processing of 0.15-1.5 ml samples with repeatable and sensitive particle separation.
  • Successful size-separation of biological samples using the integrated acoustofluidic device.
  • Optimized sample collection via real-time feedback, conserving and concentrating processed samples.

Conclusions:

  • The automated microfluidic platform provides a flexible, reliable, and precise solution for particle separation from larger sample volumes.
  • The system facilitates interchangeable device use and processing of unknown samples without extensive optimization.
  • This approach enhances the utility of microfluidics for sensitive biological sample analysis and concentration.