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Determination of Ochratoxin A in Pig Kidneys by Immunoaffinity Cleanup and Ultra-High Performance Liquid
YaLi Hou1, Jinhui Zhou, YuPing Li
1Bioengineering College of Chongqing University, Chongqing, 400030, People's Republic of China.
Abstract:
An accurate method was developed for determining ochratoxin A (OTA) in pig kidney using an immunoaffinity column for cleanup and ultra-HPLC/MS/MS for identification and quantification. Mean recoveries of OTA from kidney samples fortified at 0.10-5 μg/kg levels ranged from 74 to 92%, with RSDs of 4.6-7.5%. The LOD was estimated to be 0.03 μg/kg and the LOQ was 0.10 μg/kg based on an S/N in kidney of 3:1 and 10:1, respectively. The developed method was used for the determination of OTA in pig kidney. A survey of the OTA content of pig kidneys slaughtered in Chongqing, China, revealed that 35 out of 40 analyzed samples were contaminated; the OTA concentration in kidney ranged from trace level (0.03-0.1) to 0.323 μg/kg. This method was shown to be useful for accurately evaluating the intake of OTA from pig kidneys.
