Bianalyte multicommutated flow analysis system for microproteinuria diagnostics.

Area of Science:

• Analytical Chemistry
• Clinical Chemistry
• Biomedical Engineering

Background:

• Microproteinuria is a key indicator of kidney disease.
• Accurate and efficient methods for determining urinary protein and creatinine are crucial for diagnosis.
• Existing methods may be time-consuming or reagent-intensive.

Purpose of the Study:

• To develop and validate a novel bianalyte multicommutated flow analysis (MCFA) system.
• To enable simultaneous determination of urinary protein and creatinine for microproteinuria assessment.
• To provide an automated, economical, and reliable analytical solution.

Main Methods:

• Development of a bianalyte MCFA system utilizing two optoelectronic flow-through detectors.
• Application of the turbidimetric Exton's method for total protein determination.
• Application of the photometric Jaffe reaction for creatinine determination.

Main Results:

• The MCFA system achieved protein determination in the range of 36-300 mg L⁻¹ (detection limit 33 mg L⁻¹).
• Creatinine was determined in the range of 0.045-2.50 mmol L⁻¹ (detection limit 0.025 mmol L⁻¹).
• The system demonstrated high throughput (30 peaks/hour) and acceptable correlation with clinical analyzers when tested on real urine samples.

Conclusions:

• The developed MCFA system is a fully-mechanized, user-friendly, and reagent-economical tool.
• It offers satisfactory analytical parameters for the simultaneous determination of urinary protein and creatinine.
• The system proves analytically useful for microproteinuria assessment in clinical settings.