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Characterization of Positively Charged Lipid Shell Microbubbles with Tunable Resistive Pulse Sensing (TRPS) Method: A
Simona Manta1, Anthony Delalande2, Michel Bessodes1
1Paris Descartes University, Sorbonne Paris Cité, Team vectors for molecular imaging and targeted therapy, CNRS UTCBS UMR8258, INSERM UTCBS U1022, Chimie ParisTech, PSL Research University, Paris, France.
Tunable resistive pulse sensing (TRPS) offers a more flexible method for accurately measuring microbubble size distribution. This technique overcomes limitations of traditional methods like optical microscopy for polydisperse microparticles.
Area of Science:
- Materials Science
- Biotechnology
- Analytical Chemistry
Background:
- Microbubbles are polydisperse microparticles with challenging size distribution measurements.
- Current methods like optical microscopy, electrical sensing, and light scattering have limitations for microbubble analysis.
Purpose of the Study:
- To investigate tunable resistive pulse sensing (TRPS) as an alternative method for microbubble characterization.
- To evaluate the accuracy of TRPS for measuring microbubble concentration and size distribution.
- To compare TRPS data with results from optical microscopy.
Main Methods:
- Utilized tunable resistive pulse sensing (TRPS), a Coulter counter-based technique with adjustable parameters (flow rate, potential difference, pore size).
- Studied positively charged microbubbles.
- Performed a statistical comparison between TRPS and optical microscopy data.
Main Results:
- TRPS provides a flexible approach for microparticle analysis by allowing modulation of key parameters.
- The study details the parameters necessary for accurate microbubble concentration and size distribution measurements using TRPS.
- A statistical comparison demonstrated the performance of TRPS against optical microscopy.
Conclusions:
- TRPS is a viable and flexible technique for accurate microbubble sizing and concentration measurement.
- TRPS overcomes limitations associated with traditional methods for polydisperse microparticle analysis.
- The study provides a framework for optimizing TRPS for microbubble characterization.
