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Estimation of postmortem interval through albumin in CSF by simple dye binding method.

Ankita K Parmar1, Shobhana K Menon1

  • 1Department of Forensic Science, School of Sciences, Gujarat University, Ahmedabad-380009, India.

Science & Justice : Journal of the Forensic Science Society
|December 15, 2015
PubMed
Summary

Accurately estimating the postmortem interval is crucial for forensic investigations. This study found that cerebrospinal fluid (CSF) albumin concentration decreases linearly after death, offering a reliable method for determining time since death within a 1-4 hour error margin.

Keywords:
AlbuminBromocresol greenCerebrospinal fluidMedicolegal investigationPostmortem interval

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Area of Science:

  • Forensic Science
  • Biochemistry
  • Toxicology

Background:

  • Accurate postmortem interval estimation is vital in medicolegal cases.
  • Current methods for determining postmortem interval require refinement for greater precision.
  • Bromocresol green (BCG) is a widely used dye-binding method with potential forensic applications.

Purpose of the Study:

  • To investigate the relationship between cerebrospinal fluid (CSF) albumin concentration and postmortem interval.
  • To evaluate the utility of CSF albumin as a biomarker for estimating time since death.
  • To establish a reliable and accurate method for postmortem interval determination.

Main Methods:

  • Cerebrospinal fluid (CSF) was collected via cisternal puncture from 100 autopsy cases.
  • Albumin concentration in CSF samples was measured.
  • The correlation between albumin levels and the postmortem interval was analyzed.

Main Results:

  • A linear decrease in CSF albumin concentration was observed from 2 to 72 hours postmortem.
  • After 72 hours, albumin concentration reached 0.012 mM.
  • A significant relationship was established between albumin concentration and postmortem interval, with an error of ± 1-4 hours.

Conclusions:

  • CSF albumin concentration serves as a valuable and significant parameter for estimating the postmortem interval.
  • This method offers a precise and potentially revolutionary approach to time since death determination in forensic science.
  • The findings support the use of CSF albumin analysis in routine medicolegal investigations.