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A simple and rapid creatinine sensing via DLS selectivity, using calix[4]arene thiol functionalized gold
Pinkesh G Sutariya1, Alok Pandya2, Anand Lodha3
1Bhavans Shree I. L. Pandya Arts, Science College, Gujarat University, Dakor 388225, Gujarat, India.
Talanta
|November 24, 2015
Summary
A novel method uses calixarene-functionalized gold nanoparticles for highly sensitive creatinine detection. This approach offers a simple, rapid, and reliable tool for analyzing creatinine in human urine samples.
Area of Science:
- Nanotechnology
- Biomedical Engineering
- Analytical Chemistry
Background:
- Accurate creatinine detection is crucial for diagnosing kidney function.
- Existing methods for creatinine detection can be complex or lack sensitivity.
- Gold nanoparticles (AuNPs) offer unique optical properties for biosensing applications.
Purpose of the Study:
- To develop a simple, ultra-sensitive, and selective colorimetric method for creatinine detection.
- To functionalize gold nanoparticles with calix[4]arene for enhanced creatinine binding.
- To establish a reliable biosensor for creatinine determination in human urine.
Main Methods:
- Synthesis of gold nanoparticles and p-tert-butylcalix[4]arene using microwave-assisted methods.
- Characterization of the nanoassembly using Transmission Electron Microscopy (TEM), Dynamic Light Scattering (DLS), UV-Vis, and ESI-MS spectroscopy.
- Utilizing DLS for precise particle size determination to improve sensitivity and selectivity.
Main Results:
- The developed method achieved a low detection limit of 2.16 nM for creatinine.
- The calix[4]arene functionalized gold nanoparticles (pSDSC4-AuNPs) demonstrated strong binding affinity via hydrogen bonding and electrostatic interactions.
- The biosensor exhibited a fast response time (<60 seconds) and a long shelf-life (>5 weeks).
Conclusions:
- The pSDSC4-AuNPs based system provides a highly sensitive and selective approach for creatinine detection.
- The method shows excellent discrimination against other biomolecules, ensuring accurate results.
- This developed biosensor is a promising tool for simple, reliable, and accurate creatinine determination in clinical diagnostics.
