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Updated: Mar 28, 2026
Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
Published on: May 18, 2018
Type II arabinogalactan from Anoectochilus formosanus induced dendritic cell maturation through TLR2 and TLR4
Ching-Yi Lai1, Li-Chan Yang2, Wen-Chuan Lin3
1School of Medicine, Department of Pharmacology, China Medical University, no. 91 Hsueh Shih Road, Taichung, Taiwan, R.O.C.
Background:
Polysaccharides, considered as immunomodulators with the capacity to activate immunity against microbial pathogens and tumors, have been employed for their dietary and medical benefits.
Purpose:
This study investigated the immunomodulatory effect of polysaccharide such as type II arabinogalactan from Anoectochilus formosanus (AGAF) on dendritic cell (DC) maturation and the underlying molecular mechanisms.
Methods And Results:
Exposing DCs to AGAF induces cell maturation, which is characterized by the upregulation of CD86, CD83, CD80, CD40, and MHC class I and class II expression through flow cytometry analysis and morphological change without cytotoxicity. In addition, AGAF-triggered DC2.4 cells were involved in priming T-cell activation in vitro and in vivo. Transfection of toll-like receptor (TLR) 2 proteins and TLR4 siRNA suppressed DC maturation, suggesting that AGAF induced DC maturation through TLR2 and TLR4.
Conclusion:
These findings indicate that AGAF may be a potentially effective immunomodulator in stimulating DC maturation.
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