Related Experiment Video
Updated: Mar 28, 2026
09:36
Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases
Published on: June 28, 2019
10.8K
Chorein Sensitive Dopamine Release from Pheochromocytoma (PC12) Cells
Sabina Honisch1, Birgit Fehrenbacher, Aleksandra Lebedeva
1Department of Physiology, University of Tx00FC;bingen, Tx00FC;bingen, Germany.
Neuro-Signals
|December 15, 2015
Summary
Chorein deficiency reduces VAMP8 expression and dopamine release in neuroendocrine cells. This study investigated chorein
Area of Science:
- Neuroscience
- Cell Biology
- Biochemistry
Background:
- Chorein (VPS13A) regulates actin polymerization and cell survival, and its deficiency causes neurodegeneration.
- Chorein deficiency in platelets impairs degranulation by reducing VAMP8 expression.
- The role of chorein in neuronal cells, specifically in dopamine release, remained unclear.
Purpose of the Study:
- To investigate the role of chorein in VAMP8 expression and dopamine release in pheochromocytoma (PC12) cells.
- To determine if chorein deficiency affects vesicle dynamics and neurotransmitter release in a neuronal model.
Main Methods:
- Chorein was silenced in PC12 cells.
- Transmission electron microscopy quantified vesicle numbers.
- RT-PCR, Western blotting, and ELISA assessed VAMP8 expression and dopamine release.
Main Results:
- Chorein silencing significantly decreased vesicle count, VAMP8 transcript, and VAMP8 protein levels.
- Stimulated dopamine release by high K+ was significantly blunted in chorein-silenced cells.
- These findings indicate a critical role for chorein in regulating VAMP8 and dopamine secretion.
Conclusions:
- Chorein deficiency leads to reduced VAMP8 expression and vesicle numbers in PC12 cells.
- Chorein is essential for proper dopamine release regulation in pheochromocytoma cells.
- These findings link chorein function to neurosecretory processes relevant to neurodegenerative disorders.
More Related Videos
11:26Assessment of Dopaminergic Homeostasis in Mice by Use of High-performance Liquid Chromatography Analysis and Synaptosomal Dopamine Uptake
Published on: September 21, 2017
13.2K
07:56A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
Published on: August 11, 2021
3.9K
Related Concept Videos
01:23Cholinergic Neurons: Neurotransmission
6.2K
Cholinergic neurotransmission involves the synthesis and the release of acetylcholine (ACh) in order to transmit nerve impulses across the synapse. The process begins with the synthesis of acetyl CoA, a precursor for ACh, from ATP, acetate, and coenzyme A in the mitochondria. Choline, another vital precursor, is transported inside the neuron through choline transporters, including high-affinity choline transporter CHT1, low-affinity choline transporter CTL1, and lower-affinity choline...
6.2K
01:27Adrenergic Neurons: Neurotransmission
5.8K
Postganglionic sympathetic fibers (except those supplying the sweat glands) releasing noradrenaline or norepinephrine are called noradrenergic or adrenergic neurons. Noradrenaline, dopamine, adrenaline, or epinephrine are collectively called "catecholamines" as they contain a catechol moiety and an amine side chain. The five stages of neurotransmitter release involve their synthesis, storage, release, reuptake and metabolism.
Synthesis: Catecholamine synthesis requires tyrosine, which...
Synthesis: Catecholamine synthesis requires tyrosine, which...
5.8K
01:22Direct-Acting Cholinergic Agonists: Chemistry and Structure-Activity Relationship
2.5K
Cholinergic agonists or cholinomimetics mimic the action of acetylcholine to stimulate the parasympathetic nervous system. They are categorized into direct-acting and indirect-acting agents. The direct-acting cholinergic drugs induce the parasympathetic response by directly binding to the muscarinic or nicotine receptors. In comparison, the indirect-acting cholinergic drugs prevent acetylcholine hydrolysis, indirectly contributing to the extended parasympathetic response.
The direct-acting...
The direct-acting...
2.5K
01:21Drugs Affecting Neurotransmitter Release or Uptake
1.8K
Certain drugs can affect how neurotransmitters called catecholamines, are released or taken back up in the adrenergic neuron. They can have different effects on the body's sympathetic transmission. Reserpine, a natural compound found in the Rauwolfia shrub, blocks a transporter called vesicular monoamine transporter (VMAT), which leads to a buildup of catecholamines in the cell and reduces sympathetic transmission. Another drug called guanethidine works in multiple ways, including blocking...
1.8K
01:29Drugs Affecting Neurotransmitter Synthesis
2.5K
Drugs affecting neurotransmitter synthesis can impact the adrenergic neuron and the synthesis of neurotransmitters. For example, α-methyltyrosine and carbidopa target specific enzymes involved in catecholamine synthesis. α-methyltyrosine inhibits the enzyme tyrosine hydroxylase, which converts tyrosine into dopamine. By blocking this enzyme, α-methyltyrosine reduces dopamine production and other catecholamines. Carbidopa, on the other hand, inhibits the enzyme dopa decarboxylase,...
2.5K
01:31Direct-Acting Cholinergic Agonists: Pharmacokinetics
2.0K
Direct-acting cholinergic agonists, such as synthetic choline esters and naturally occurring alkaloids, exert their effects by enhancing the actions of acetylcholine and stimulating the parasympathetic nervous system. Synthetic choline esters share structural similarities with acetylcholine. For example, they have a positively charged quaternary ammonium or onium group, contributing to their hydrophilic characteristics. As a result, they are poorly absorbed in the body through oral...
2.0K