Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

4.4K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
4.4K
Stem Cell Therapy for Tissue Regeneration01:21

Stem Cell Therapy for Tissue Regeneration

4.9K
Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
4.9K
Bone Marrow Sampling and Transplants01:22

Bone Marrow Sampling and Transplants

2.4K
Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy...
2.4K
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

4.1K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
4.1K
Hematopoiesis01:21

Hematopoiesis

9.8K
The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
9.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Moving from 'stigma reduction' to 'inclusion': development of the inclusion collaborative at Nepean Blue Mountains Local Health District, New South Wales.

Harm reduction journal·2024
Same author

Carbapenemase-producing enterobacterales colonisation status does not lead to more frequent admissions: a linked patient study.

Antimicrobial resistance and infection control·2024
Same author

Lipidomic Risk Score to Enhance Cardiovascular Risk Stratification for Primary Prevention.

Journal of the American College of Cardiology·2024
Same author

Defined synthetic microbial communities colonize and benefit field-grown sorghum.

The ISME journal·2024
Same author

DDX41 haploinsufficiency causes inefficient hematopoiesis under stress and cooperates with p53 mutations to cause hematologic malignancy.

Leukemia·2024
Same author

Living evidence syntheses: the emerging opportunity to increase evidence-informed health policy in Australia.

The Medical journal of Australia·2024
Same journal

A tri-axis optomechanical accelerometer with plasmonic MIM waveguide and structural direction-dependent optical signatures.

Scientific reports·2026
Same journal

Holographic leaky-wave antennas with independently controlled multiple counter-rotating vortex beams.

Scientific reports·2026
Same journal

Differential associations of longitudinal hearing and vision trajectories with dementia and mild cognitive impairment in older adults.

Scientific reports·2026
Same journal

Abdominal obesity and leisure-time sedentary behavior in relation to gastroesophageal reflux disease risk: a prospective cohort study from the UK Biobank.

Scientific reports·2026
Same journal

Effect of nitrogen-rich COF incorporation on the structure and separation performance of polyamide nanofiltration membranes.

Scientific reports·2026
Same journal

Withanolide A inhibits hIAPP aggregation: An In silico, biophysical, and drosophila-based In vivo validation.

Scientific reports·2026
See all related articles

Related Experiment Video

Updated: Mar 28, 2026

Author Spotlight: Exploring the Lifespan Dynamics of Healthy Human Hematopoiesis
03:40

Author Spotlight: Exploring the Lifespan Dynamics of Healthy Human Hematopoiesis

Published on: December 8, 2023

2.0K

Fancb deficiency impairs hematopoietic stem cell function.

Wei Du1,2, Surya Amarachintha1, Ozlem Erden1

  • 1Division of Experimental Hematology and Cancer Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio 45229.

Scientific Reports
|December 15, 2015
PubMed
Summary
This summary is machine-generated.

Fanconi anemia (FA) is a genetic disorder. This study shows that Fancb deficiency in mice impairs hematopoietic stem cell (HSC) function and bone marrow recovery, offering insights into FA pathogenesis.

More Related Videos

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
10:25

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells

Published on: August 9, 2019

10.2K
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
09:01

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

Published on: March 26, 2018

14.9K

Related Experiment Videos

Last Updated: Mar 28, 2026

Author Spotlight: Exploring the Lifespan Dynamics of Healthy Human Hematopoiesis
03:40

Author Spotlight: Exploring the Lifespan Dynamics of Healthy Human Hematopoiesis

Published on: December 8, 2023

2.0K
Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
10:25

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells

Published on: August 9, 2019

10.2K
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
09:01

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

Published on: March 26, 2018

14.9K

Area of Science:

  • Hematology
  • Genetics
  • Molecular Biology

Background:

  • Fanconi anemia (FA) is a rare genetic disorder causing bone marrow failure, congenital abnormalities, and cancer predisposition.
  • FANCB (FAAP95) is the sole X-linked gene implicated in FA, highlighting a gap in understanding X-linked FA mechanisms.
  • Hematopoietic stem cell (HSC) dysfunction is central to FA pathogenesis, but the specific role of FANCB in HSC maintenance remains unclear.

Purpose of the Study:

  • To investigate the role of FANCB in adult hematopoiesis using a mouse model.
  • To characterize the impact of Fancb deficiency on hematopoietic stem cell (HSC) function and bone marrow recovery.
  • To explore the molecular mechanisms underlying Fancb deficiency-related HSC defects.

Main Methods:

  • Hematopoietic stem cell (HSC) and progenitor cell analysis in adult Fancb-deficient (Fancb(-/y)) mice.
  • Assessment of HSC quiescence, in vitro progenitor activity, and in vivo repopulating capacity.
  • Evaluation of bone marrow sensitivity to DNA cross-linking agents (mitomycin C) and myelotoxic stress (5-fluorouracil).
  • RNA sequencing (RNA-seq) analysis of Fancb(-/y) HSC and progenitor cells to identify gene expression alterations.

Main Results:

  • Fancb(-/y) mice exhibited decreased HSC quiescence, reduced progenitor activity, and impaired in vivo repopulating capacity.
  • The hematopoietic system of Fancb(-/y) mice showed hypersensitivity to mitomycin C, leading to bone marrow failure.
  • Fancb(-/y) bone marrow demonstrated slower recovery and reduced tolerance to 5-fluorouracil-induced myelotoxic stress.
  • RNA-seq analysis revealed dysregulated expression of genes critical for HSC function and cell cycle regulation in Fancb(-/y) cells.

Conclusions:

  • Fancb deficiency critically impairs HSC function and bone marrow maintenance.
  • The Fancb(-/y) mouse model is valuable for studying the FA pathway's role in HSC homeostasis.
  • This model offers new avenues for understanding FA pathogenesis and potential therapeutic targets, particularly concerning X-linked inheritance.