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Efficacy of doxycycline release collagen membrane on surgically created and contaminated defects in rat tibiae: A
Esma Kütan1, Gonca Duygu-Çapar2, Ceyda Özçakir-Tomruk3
1Department of Oral Implantology, Faculty of Dentistry, Yeditepe University,Göztepe, Istanbul, Turkey.
Background:
The effects of systemic antibiotics on controlling infective pathogens after guided bone regeneration(GBR) procedures especially in membrane exposures are limited. However, local administrations of antibiotics are rare in GBR techniques.
Aim:
The aim of this study was to investigate the osteogenesis potential and the antibacterial effect of a doxycycline releasing collagen membrane in surgically created and contaminated defects in rat tibiae.
Material And Methods:
Defects were created in 20 rats that were randomly divided in to two groups: control group (defect contaminated by Porphyromonas gingivalis, filled with bone graft and covered by collagen membrane); test group (defect contaminated by P. gingivalis filled with bone graft and covered by collagen membrane containing 1mg/cm(2) doxycycline. Animals were sacrificed post surgically on the 14th day for microbiologic evaluation and on the 28th day for histopathological evaluation.
Results:
The degree of osteogenesis in the test group was seen to be significantly higher than control group (p: 0.011; p<0.05). Furthermore in test group, no bacterial growth was observed. The bacteria counts were determined between 1×104 and 268×104CFU/g with a median of 1.32×104 for control group.
Conclusions:
Within the limitations of this study, the results of the present study suggests that the use of a doxycycline releasing membrane has a positive effect on contaminated GBR procedures for limiting P. gingivalis infections leading to bone formation following GBR procedures in a rat model.
Insights
A doxycycline-releasing collagen membrane enhanced bone formation and prevented infection in guided bone regeneration (GBR) procedures. This localized antibiotic delivery offers a promising solution for contaminated GBR defects, promoting better osteogenesis.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Periodontology
Background:
- Systemic antibiotics have limited efficacy in controlling pathogens during guided bone regeneration (GBR), particularly with membrane exposure.
- Local antibiotic delivery in GBR is an underutilized approach.
Purpose of the Study:
- To evaluate the osteogenesis potential and antibacterial efficacy of a doxycycline-releasing collagen membrane.
- To assess its impact on surgically created and contaminated defects in a rat tibia model.
Main Methods:
- Surgically created defects in 20 rats were contaminated with Porphyromonas gingivalis.
- Defects were grafted and covered with either a standard collagen membrane (control) or a doxycycline-releasing collagen membrane (test group).
- Microbiologic and histopathological evaluations were performed on days 14 and 28, respectively.
Main Results:
- The doxycycline-releasing membrane group demonstrated significantly higher osteogenesis compared to the control group (p=0.011).
- No bacterial growth was detected in the test group.
- Bacterial counts in the control group ranged from 1x10^4 to 268x10^4 CFU/g.
Conclusions:
- Doxycycline-releasing membranes positively impact contaminated GBR procedures by limiting P. gingivalis infections.
- This localized delivery promotes bone formation in GBR, as evidenced in a rat model.
- The findings suggest a potential benefit for doxycycline-releasing membranes in managing GBR complications.
