Related Experiment Video
Updated: Mar 28, 2026

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
Published on: June 24, 2019
Dynamics of the Plant Nuclear Envelope During Cell Division
David E Evans1, Katja Graumann2
1Department of Biological and Medical Sciences, Faculty of Health and Life Sciences, Oxford Brookes University, Headington Campus, Oxford, OX3 0BP, UK. deevans@brookes.ac.uk.
Abstract:
The use of suspension cultures synchronised by aphidicolin provides a method to study cell division in living plant cells. This chapter describes the use of this technique in tobacco suspension cultures expressing nuclear and nuclear envelope proteins that have been fused to fluorescent proteins. The protocol provides advice on optimizing synchrony and on real-time imaging by confocal microscopy.
Related Concept Videos
The Phragmoplast
The...
The Phragmoplast
Determining the Plane of Cell Division
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division...
Determining the Plane of Cell Division
Mitosis and Cytokinesis
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...
Mitosis and Cytokinesis
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...

