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Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
Published on: April 14, 2016
Complete nucleotide sequence of the 16S rRNA from Lactobacillus paracasei HS-05 isolated from women's hands
Woon Yong Choi1, Hyeon Yong Lee2
1Department of Biomedical Materials Engineering, Kangwon National University, Chuncheon, 200-701, Korea. cwy1012@hanmail.net.
Abstract:
We determined the complete nucleotide sequence of the 16S rRNA from a new bacterium collected from the surfaces of women's hands. We also compared the presence of various bacteria based on the subjects' sex and age. The number of colonies isolated from the hand surface was larger for women than men, and the largest number of isolates was confirmed to be present for the women in their 30 s and men in their 40 s (147 and 34 isolates, respectively). The morphology of an isolated bacterial strain was found to be rod type, and the bacterium was identified as Lactobacillaceae species based on the GenBank database, through a phylogenetic analysis using the 16S rRNA sequence. Based on the results of a homology search, the isolated strain was 99 % identical to Lactobacillus paracasei, so it was designated Lactobacillus paracasei HS-05 and was registered in the Korea Culture Center of Microorganisms (KCCM) database as [KCCM11349P].

