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Related Concept Videos

Tissues01:18

Tissues

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Cells with similar structure and function are grouped into tissues. A group of tissues with a specialized function is called an organ. There are four main types of tissue in vertebrates: epithelial, connective, muscle, and nervous.
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DNA Microarrays02:34

DNA Microarrays

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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A Next-generation Tissue Microarray ngTMA Protocol for Biomarker Studies
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A Next-generation Tissue Microarray ngTMA Protocol for Biomarker Studies

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Tissue Microarrays.

Ana-Maria Dancau1, Ronald Simon1, Martina Mirlacher1

  • 1Institute of Pathology, University Medical Center Hamburg-Eppendorf, Martinistraße 52, Hamburg, 20246, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|December 16, 2015
PubMed
Summary
This summary is machine-generated.

Tissue microarray (TMA) technology enables high-throughput in situ analysis of numerous clinical tissue samples. This method standardizes and accelerates the validation of genes identified through next-generation sequencing for disease research.

Keywords:
FISHFluorescence in situ hybridizationHigh-throughput in situ analysisIHCImmunohistochemistryTMATissue microarraysTranslational research

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Area of Science:

  • Biotechnology
  • Genomics
  • Pathology

Background:

  • Next-generation sequencing and microarray technologies identify numerous disease-associated genes at DNA, RNA, and methylation RNA levels.
  • Validating these candidate genes requires in situ analysis of extensive clinical tissue samples.

Purpose of the Study:

  • To introduce and highlight the utility of tissue microarray (TMA) technology for efficient and standardized in situ analysis of clinical tissues.
  • To demonstrate how TMA facilitates the validation of candidate genes identified by high-throughput molecular profiling.

Main Methods:

  • Tissue microarray (TMA) construction involves assembling minute tissue cores (0.6 mm) from up to 1000 donors onto a single microscope slide.
  • Standard in situ hybridization techniques, including immunohistochemistry, fluorescence in situ hybridization, and RNA in situ hybridization, are applicable to TMAs.
  • Simultaneous analysis of all tissues on a single slide using identical reagent batches ensures high standardization.

Main Results:

  • TMA technology significantly accelerates the validation process for a large number of candidate genes.
  • The method offers high throughput, cost-efficiency, and standardization in molecular pathology studies.
  • TMAs enable simultaneous in situ analysis across diverse tissue types, enhancing data comparability.

Conclusions:

  • Tissue microarray technology is a powerful tool for the in situ validation of candidate genes identified through large-scale molecular studies.
  • TMAs provide a standardized, rapid, and cost-effective approach for analyzing numerous clinical tissue samples.
  • This technology is crucial for advancing research in cancer and other diseases by facilitating comprehensive gene validation.