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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

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MicroRNA (miRNA) Profiling.

Lu Gao¹, Feng Jiang²

¹Department of Pathology, University of Maryland School of Medicine, 10 South Pine Street, MSTF 7th Floor, Baltimore, MD, 21201-1192, USA.

Methods in Molecular Biology (Clifton, N.J.)

|December 16, 2015

Summary

This summary is machine-generated.

MicroRNAs (miRNAs) are key gene regulators impacting cell processes and disease. This review details essential miRNA expression profiling techniques, highlighting their benefits and limitations for research and clinical use.

Keywords:

DDPCR Microarray miRNA

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Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

MicroRNAs (miRNAs) are small noncoding RNAs regulating gene expression.
They control fundamental cellular processes like proliferation, apoptosis, and development.
miRNAs hold potential as diagnostic and therapeutic targets for various diseases.

Purpose of the Study:

To review and discuss common techniques for miRNA expression profiling.
To analyze the advantages and restrictions of each profiling method.
To provide a guide for selecting appropriate miRNA profiling strategies.

Main Methods:

Review of established miRNA expression profiling methodologies.
Comparative analysis of techniques based on sensitivity, specificity, and cost.
Discussion of data normalization and interpretation challenges.

Main Results:

Several miRNA profiling techniques exist, each with unique strengths and weaknesses.
The choice of method depends on the research question, sample type, and available resources.
Accurate miRNA profiling is crucial for understanding miRNA function and disease association.

Conclusions:

Effective miRNA expression profiling is vital for advancing miRNA research.
Understanding the nuances of different techniques is essential for reliable results.
This review aids researchers in selecting optimal methods for miRNA studies and clinical applications.