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The location of DNA in RecA-DNA helical filaments
1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511.
Science (New York, N.Y.)
|July 28, 1989
Abstract:
The helical filament that the RecA protein of Escherichia coli forms around DNA is the active apparatus in protein-catalyzed homologous genetic recombination. The actual position of DNA within this complex has been unknown. Image analysis has been performed on electron micrographs of filaments of RecA on double-stranded DNA and on single-stranded DNA to visualize a difference that is consistent with one strand of the double-stranded DNA. This localization of the DNA gives additional information about the unusual structure of DNA in the complex with RecA protein.