Related Experiment Video
Updated: Mar 28, 2026

Genomic Transformation of the Picoeukaryote Ostreococcus tauri
Published on: July 13, 2012
Transformation of the yeast Trichosporonoides oedocephalis
Liangzhi Li1,2, Tianyi Yang3, Chao Hu3,4
1School of Chemistry and Bioengineering, Suzhou University of Science and Technology, Suzhou, 215009, People's Republic of China. liliangzhi01@163.com.
Abstract:
The osmotolerant yeast, Trichosporonoides oedocephalis, is an excellent producer of erythritol, which has wide industrial applications. In this study, we developed an efficient transformation method for T. oedocephalis. To evaluate the T. oedocephalis transformation, we constructed a DNA fragment (loxP-Kan-loxP/Cre system) that was targeted to the mitogen-activated protein kinase HOG1 gene. Transformants were selected on plates containing G418 and response surface methodology was employed to obtain optimum transformation conditions. Optimal transformation could be achieved at an incubation time of 40 min, when the concentration of zymolyase-100T was 30 µg/mL, and when 100 mM CaCl2 was added to the mixture. The predicted optimal transformation efficiency was 133 transformants per µg of DNA. This novel method will facilitate studies in synthetic biology and metabolic engineering of T. oedocephalis.
More Related Videos
12:12Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice
Published on: February 8, 2016
07:32Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans
Published on: March 19, 2015
Related Concept Videos
Fungal Phylum Microsporidia
Fungal Phylum Ascomycota
Fungal Group Zygomycota
Bioreactor Controls-III