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Atypical Pneumonia - Screening in a Tertiary Care Centre
Aruna Dorairaj1, Sridharan Sathyamoorthy Kopula2, Kennedy Kumar2
1Post Graduate Student, Department of Microbiology, Sri Ramachandra Medical College and Research Institute, Sri Ramachandra University , Porur, Chennai, India .
Introduction:
Pneumonia is one of the leading infectious causes of mortality and morbidity worldwide. Atypical respiratory pathogens account for 30 - 40% of these infections. The three most important atypical pathogens are Mycoplasma pneumoniae, Chlamydophila pneumoniae and Legionella pneumophila.
Aim:
To screen for atypical pathogens as cause for community acquired pneumonia.
Materials And Methods:
A cross-sectional study was done with 107 patients who had clinical suspicion of atypical pneumonia. The presence of atypical pathogens Mycoplasma pneumoniae, Chlamydophila pneumoniae and Legionella pneumophila were screened from the patient's sample. Respiratory samples in the form of sputum, Broncheoalveolar lavage and Non- Broncheoalveolar lavage were used for cultivation of Mycoplasma pneumoniae and Legionella pneumophila. Urine specimen was used for the detection of Legionella antigen. Serum samples were used for the detection of Mycoplasma pneumoniae IgM and Chlamydophila pneumoniae IgM antibodies.
Results:
Among the 107 samples screened, 13(12.1%) were positive for antibodies against atypical pathogens. Out of which 7(6.5%) had IgM antibodies against Mycoplasma pneumoniae and the rest 6(5.6%) had Chlamydophila pneumoniae IgM antibodies. All the samples were culture negative for Mycoplasma pneumoniae and Legionella pneumophila. Urinary antigen detection for Legionella pneumophila was also negative in patients.
Conclusion:
Atypical pathogens should also be considered routinely as a cause of lower respiratory tract infections, especially Chlamydia pneumoniae and Mycoplasma pneumoniae in our country. Serological diagnosis by ELISA can be adopted for determining the infections by atypical pathogens as cultivation is difficult and also requires special media and prolonged incubation. This may not be feasible for many laboratories. Early diagnosis and treatment will reduce the complications.
Insights
Atypical pathogens like Mycoplasma pneumoniae and Chlamydophila pneumoniae are common causes of community-acquired pneumonia. Serological tests are effective for diagnosing these infections when cultures are difficult.
Area of Science:
- Infectious Diseases
- Microbiology
- Clinical Diagnostics
Background:
- Pneumonia remains a significant global cause of mortality and morbidity.
- Atypical respiratory pathogens contribute to 30-40% of pneumonia cases.
- Key atypical pathogens include Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella pneumophila.
Purpose of the Study:
- To investigate the prevalence of atypical pathogens in community-acquired pneumonia.
- To evaluate diagnostic methods for atypical pneumonia.
Main Methods:
- A cross-sectional study involved 107 patients with suspected atypical pneumonia.
- Samples analyzed included sputum, bronchoalveolar lavage, urine, and serum.
- Detection methods comprised bacterial culture, urinary antigen tests, and IgM antibody assays (ELISA).
Main Results:
- 12.1% of patients tested positive for atypical pathogen antibodies.
- Mycoplasma pneumoniae IgM antibodies were detected in 6.5% and Chlamydophila pneumoniae IgM in 5.6%.
- All cultures and Legionella pneumophila urinary antigen tests were negative.
Conclusions:
- Atypical pathogens, particularly Mycoplasma pneumoniae and Chlamydophila pneumoniae, are important causes of lower respiratory tract infections.
- Serological diagnosis using ELISA is a practical alternative to difficult cultivation methods.
- Early detection and treatment of atypical pneumonia can prevent complications.
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