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High Resolution Physical Characterization of Single Metallic Nanoparticles

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New Tools for Characterizing Metallic Nanoparticles: AgNPs, A Case Study.

Rodrigo A González-Fuenzalida¹, Yolanda Moliner-Martínez¹, Carmen Molins-Legua¹

¹MINTOTA Research Group. Departament de Química Analítica, Facultat de Química, Universitat de Valencia , C/Doctor Moliner, 50, 46100 Burjassot, Valencia, Spain.

Analytical Chemistry

|December 18, 2015

Summary

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This study introduces capillary liquid chromatography (Cap-LC) as a novel method for determining silver nanoparticle (AgNP) size and concentration, offering an alternative to transmission electron microscopy (TEM). The technique shows high accuracy, with relative errors up to 5% compared to TEM.

Area of Science:

Nanotechnology
Analytical Chemistry
Materials Science

Background:

Transmission electron microscopy (TEM) is the standard for nanoparticle size and concentration estimation.
Accurate characterization of nanoparticles (NPs) is crucial for understanding their properties and applications.

Purpose of the Study:

To demonstrate the potential of C18 reversed-phase capillary liquid chromatography (Cap-LC) coupled to diode array detection (DAD) for estimating silver nanoparticle (AgNP) size and concentration.
To establish Cap-LC-DAD as a viable alternative to TEM for NP characterization.

Main Methods:

Direct sample injection into C18 reversed-phase Cap-LC-DAD without prior extraction.
Calibration using a single standard with known AgNP size.
Coupling with in-tube solid-phase microextraction (IT-SPME) for studying particle distribution and hydrodynamic diameter.

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Main Results:

Cap-LC-DAD accurately estimated AgNP size and concentration, with relative errors up to 5% compared to TEM.
The method is applicable to AgNPs synthesized via different methods and their aqueous dilutions.
IT-SPME-Cap-LC enabled the study of particle distribution by polarity and response to changes in hydrodynamic diameter, applicable to both AgNPs and gold nanoparticles (AuNPs).

Conclusions:

C18 reversed-phase Cap-LC-DAD is a promising technique for estimating AgNP size and concentration, complementing or potentially replacing TEM.
The method is versatile, applicable to various AgNP types and can be extended to other nanoparticles like AuNPs.
IT-SPME-Cap-LC offers enhanced capabilities for studying NP properties, including size-dependent distribution and interactions with surfactants.