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Procedures for Rat in situ Skeletal Muscle Contractile Properties
Published on: October 15, 2011
Proteolytic enzyme activities in skeletal muscle of high jumping rats
I Sohár1, S Hatfaludy, J Rohán
1Department of Biochemistry, A. Szent-Gyorgyi Medical University, Szeged, Hungary.
Abstract:
Physical activity can be studied by various kind of exercises. Many works have been published in field of proteolytic enzyme activities in skeletal muscle during endurance training. In this work we used high jumping as a dynamic force velocity training to study the changes in proteolytic enzyme activities during this type of exercise. The activity of cathepsin D, cathepsin L and ATN-ase (Acetyl-Tyrosine-paranitroanilide-splitting enzyme) in vastus lateralis muscle was measured after one, 3, 7 or 11 weeks of high jumping exercise. The results demonstrated that proteinase activity began to increase when the load, i.e. number of jumping and the weight put on the rat's back was too much for their muscles. They could carry out the task consuming the energy originating from muscle tissue in the first period of the experiment, but in the second period (after 7 weeks) the type of training with this load became equal with an endurance training.
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