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Updated: Aug 17, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)
Published on: March 14, 2013
High-performance liquid chromatography/mass spectrometry: state of the art for the drug analysis laboratory
1Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis 55455.
Abstract:
The combination of HPLC and mass spectrometry has great promise for the toxicology laboratory. In the past five years, significant progress has been made toward producing a reliable interface for these techniques. Thermospray, liquid ion evaporation, ion-spray, and the particle beam separator are all viable "second generation" approaches with significant advantages and disadvantages. I review the operation of these interfaces with an orientation to their use in the drug analysis laboratory. Thermospray, ion-spray, and liquid ion evaporation primarily supply information about molecular mass. The benefits and limitations of using tandem mass spectrometry to obtain additional structural information will also be discussed.
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