The complete chloroplast genome of the southern Chinese pine Pinus tabuliformis (Pinales: Pinaceae)
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Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
Zhongdong Yu1, Shaobing Peng1, Peihua Yang1
1a College of Forestry, Northwest A&F University , Yangling , China.
Abstract:
The complete nucleotide sequence of Pinus tabuliformis chloroplast genome (cpDNA) was carried out using Illumina Hiseq 2500. The genome is 119 646 bp in length, and the overall base composition of H-strand is 30.6% for A, 19.3% for C, 19.2% for G and 30.9% for T. There are 116 genes in the cpDNA, including 74 protein-coding genes, four ribosomal RNA genes (four rRNA species), and 36 transfer RNA genes (20 tRNA species). Phylogenetic analyses of P. tabuliformis and other 12 species belonging to Pinales were carried out using MEGA 6.0 with Neighbor-Joining methods. The sequences of P. tabuliformis were clustered in subfamily Pinaceae.
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