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Related Experiment Video

Updated: Mar 28, 2026

Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil Ers4tU

Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil Ers4tU

Published on: August 22, 2019

Transcriptome-wide RNA processing kinetics revealed using extremely short 4tU labeling.

J David Barrass¹, Jane E A Reid¹, Yuanhua Huang²

¹Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, EH9 3BF, UK.

|December 19, 2015

Summary

This summary is machine-generated.

Researchers improved RNA labeling in yeast to detect nascent transcription. This allowed analysis of short-lived RNAs and pre-mRNA splicing, revealing links between RNA stability, structure, and splicing efficiency.

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Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

Published on: October 22, 2018

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Last Updated: Mar 28, 2026

Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil Ers4tU

Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil Ers4tU

Published on: August 22, 2019

Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture

Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture

Published on: August 8, 2013

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

Published on: October 22, 2018

Area of Science:

Molecular Biology
Yeast Genetics
RNA Metabolism

Background:

Cellular RNA levels result from complex synthesis, decay, and processing dynamics.
Metabolic labeling with nucleotide analogs offers a global approach to study these RNA processes.

Purpose of the Study:

To enhance RNA labeling techniques for high-resolution kinetic analysis in Saccharomyces cerevisiae.
To investigate nascent transcription, short-lived non-coding RNAs, and pre-mRNA splicing kinetics.

Main Methods:

Improved 4-thiouracil labeling of RNA in yeast.
Isolation and analysis of nascent RNA labeled for short durations (1-5 minutes).
Application of reverse transcriptase-quantitative polymerase chain reaction and RNA sequencing.

Main Results:

Detection of nascent pervasive transcription and short-lived non-coding RNAs.
Analysis of intron-containing pre-mRNAs in wild-type yeast.
Measurement of pre-mRNA stability and correlation with sequence features.

Conclusions:

Significant association found between non-coding RNA stability, transcript length, and predicted secondary structure.
Efficient splicing of ribosomal protein transcripts correlates with less stable intron secondary structures.
Optimal intron secondary structure stability facilitates rapid pre-mRNA splicing.