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Updated: Mar 28, 2026
Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain
Published on: March 13, 2020
Chromatographic analysis of wheatgrass extracts
Masood Shah Khan1, Rabea Parveen2, Kshipra Mishra3
1Department of Pharmacognosy and Phytochemistry, Bioactive Natural Product Laboratory, Jamia Hamdard, Uttar Pradesh, India ; Department of Pharmacy, Faculty of Science, Mohammad Ali Jauhar University, Rampur, Uttar Pradesh, India.
Developed high-performance thin layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC) methods enable quality control for wheatgrass (WG) extracts. These validated techniques ensure the standardization of WG nutritional supplements.
Area of Science:
- Phytochemistry
- Analytical Chemistry
- Nutraceuticals
Background:
- Wheatgrass (Triticum aestivum Linn.) is rich in chlorophyll, vitamins, minerals, enzymes, and amino acids.
- It exhibits potential anti-cancer, anti-ulcer, antioxidant, and anti-arthritic properties.
- Standardized quality evaluation is crucial for its use as a nutritional supplement.
Purpose of the Study:
- To develop and validate high-performance thin layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC) methods.
- To enable qualitative and quantitative analysis of wheatgrass (WG) extracts.
- To facilitate the quality control and standardization of WG.
Main Methods:
- Sample preparation involved sonication in methanol and water.
- HPTLC analysis utilized pre-coated silica plates and specific mobile phases.
- HPLC analysis was performed using a Waters system with a Merck C18 column and a defined mobile phase.
Main Results:
- HPTLC fingerprinting revealed 10-11 spots at various wavelengths.
- HPLC fingerprinting generated 22 peaks at 256 nm.
- Quantitative analysis determined gallic acid content at 0.077% (HPTLC) and 0.07% (HPLC), and rutin at 0.04% (HPLC) in aqueous WG extract.
Conclusions:
- The developed HPTLC and HPLC methods are suitable for quality control.
- These methods allow for the standardization of wheatgrass (WG) and its extracts.
- Ensures consistent quality for WG-based nutritional supplements.
