Related Experiment Video
Updated: Mar 28, 2026

Author Spotlight: Photo Switchable Protein Recruitment for Reversible Patterning in Artificial Cellular Systems
Published on: February 23, 2024
Light-Activated Reversible Imine Isomerization: Towards a Photochromic Protein Switch
Tetyana Berbasova1, Elizabeth M Santos1, Meisam Nosrati1
1Department of Chemistry, Michigan State University, East Lansing, MI, 48824, USA.
Abstract:
Mutants of cellular retinoic acid-binding protein II (CRABPII), engineered to bind all-trans-retinal as an iminium species, demonstrate photochromism upon irradiation with light at different wavelengths. UV light irradiation populates the cis-imine geometry, which has a high pKa , leading to protonation of the imine and subsequent "turn-on" of color. Yellow light irradiation yields the trans-imine isomer, which has a depressed pKa , leading to loss of color because the imine is not protonated. The protein-bound retinylidene chromophore undergoes photoinduced reversible interconversion between the colored and uncolored species, with excellent fatigue resistance.
More Related Videos
06:41Building a Simple and Versatile Illumination System for Optogenetic Experiments
Published on: January 12, 2021
09:32Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
Published on: June 15, 2017
Related Concept Videos
Channel Rhodopsins
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
Photoreceptors and Visual Pathways