Related Experiment Video
Updated: Mar 28, 2026
Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture
Published on: October 28, 2022
Sterilization of auto-crosslinked hyaluronic acid scaffolds structured in microparticles and sponges
Andréa Arruda Martins Shimojo1, Isabela Cambraia de Souza Brissac1, Lucas Martins Pina1
1Department of Engineering of Materials and Bioprocesses, School of Chemical Engineering, University of Campinas, Campinas-SP, Brazil.
Abstract:
This work evaluated the effects of UV irradiation, plasma radiation, steam and 70% ethanol treatments on the sterilization and integrity of auto-crosslinked hyaluronic acid (HA-ACP) scaffolds structured in microparticles and sponges aiming in vivo applications for regenerative medicine of bone tissue. The integrity of the microparticles was characterized by rheological behavior, while for the sponges, it was characterized by scanning electron microscopy, Fourier transform infrared spectroscopy and differential scanning calorimetry. The effectiveness of the sterilization treatment was verified by the number of microorganism colonies in the samples after the treatments. In conclusion, plasma radiation was the best treatment for the sponges, while steam sterilization in the autoclave at 126°C (1.5 kgf/cm2) for 5 min was the best treatment for the microparticles.
